Glycoprotein E of bovine herpesvirus type 1 is involved in virus transmission by direct cell-to-cell spread

Xavier Rebordosa, Jaume Piñol, Josep A. Pérez-Pons, Jorge Lloberas, Jordi Naval, Xavier Serra-Hartmann, Enric Espuña, Enrique Querol

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43 Citations (Scopus)

Abstract

In order to identify the role of the bovine herpesvirus type 1 (BHV-1) glycoprotein E (gE) in the viral infection cycle, we have constructed a BHV-1 gE deletion mutant strain (BHV-1 gE-). This strain was assayed in vitro by comparing its growth kinetics with the wild type strain used as a host of the deletion. Our results indicate that those conditions which prevent the infection by direct adsorption to the cells (presence of a semi-solid medium or presence of neutralizing antibodies in the medium) selectively inhibit the growth of the gE- strain, suggesting that gE plays a central role in the BHV-1 spread by direct cell-to-cell transmission, a major mechanism of the BHV-1 in vivo virulence.
Original languageEnglish
Pages (from-to)59-68
JournalVirus Research
Volume45
Issue number1
DOIs
Publication statusPublished - 1 Jan 1996

Keywords

  • Bovine herpesvirus type 1
  • Cell-to-cell spread
  • Deletion mutant
  • Glycoprotein E
  • Infectious bovine rhinotracheitis

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