Glucose sensor based on an amperometric microelectrode with a photopolymerizable enzyme membrane

C. Jiménez, J. Bartrolí, N. F. de Rooij, M. Koudelka-Hep

Research output: Contribution to journalArticleResearchpeer-review

13 Citations (Scopus)

Abstract

An amperometric glucose sensor based on a photocurable polyacrylamide membrane, in which the enzyme glucose oxidase is entrapped, is reported. The enzymatic membrane is deposited on a planar three-microelectrode cell by casting and by photolithography patterning. The latter technique allows us to obtain reproducible and thin membranes. The glucose sensors developed present a sensitivity from 45 to 67 nA mM-1 glucose depending on the membrane thickness and the enzyme concentration in a linear range of 4 × 10-3-1 mM. Sensors with a 10-15 μm membrane thickness exhibit a short response time of 20-30 s (t95%) and a long-term stability of three weeks. © 1995.
Original languageEnglish
Pages (from-to)421-424
JournalSensors and Actuators: B. Chemical
Volume27
Issue number1-3
DOIs
Publication statusPublished - 1 Jan 1995

Keywords

  • Enzyme sensors
  • Glucose sensors
  • Photopolymers

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