Glucose sensor based on an amperometric microelectrode with a photopolymerizable enzyme membrane

An amperometric glucose sensor based on a photocurable polyacrylamide membrane, in which the enzyme glucose oxidase is entrapped, is reported. The enzymatic membrane is deposited on a planar three-microelectrode cell by casting and by photolithography patterning. The latter technique allows us to obtain reproducible and thin membranes. The glucose sensors developed present a sensitivity from 45 to 67 nA mM-1 glucose depending on the membrane thickness and the enzyme concentration in a linear range of 4 × 10-3-1 mM. Sensors with a 10-15 μm membrane thickness exhibit a short response time of 20-30 s (t95%) and a long-term stability of three weeks. © 1995.