An amperometric glucose biosensor strip based on a three electrodes (counter, reference and working) planar configuration was fabricated using thick film technology. Glucose oxidase (GOD) was immobilized in the bulk of a graphite-epoxy composite paste. This paste was used to screen-print the working electrode on a glass fibre board. The integration on the same support of pseudo-reference electrode was obtained by screen printing a commercial silver-epoxy paste, and subsequent electrochemical chlorinization in KCl 1 M at + 1 V (SCE). Finally, the counter electrode was integrated using the same technique by depositing a graphite-epoxy composite paste, taking into account the counter/working electrode area ratio. Response characteristics of a glucose biosensor strip based on this transducer are similar to those of previously reported one electrode configuration glucose biosensor constructed using the same biocomposite material [Galan-Vidal and Munoz, Sens. Actuators B 45 (1997) 55-62].