Gluconeogenesis-linked glycogen metabolism is important in the achievement of in vitro capacitation of dog spermatozoa in a medium without glucose

J. L. Albarracín, J. M. Fernández-Novell, J. Ballester, M. C. Rauch, A. Quintero-Moreno, A. Peña, T. Mogas, T. Rigau, A. Yañez, J. J. Guinovart, J. C. Siebe, I. I. Concha, Juan Enrique Rodriguez Gil

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Abstract

In vitro capacitation of dog spermatozoa in a medium without sugars and with lactate as the metabolic substrate (I-CCM) was accompanied by a progressive increase of intracellular glycogen during the first 2 h of incubation, which was followed by a subsequent decrease of glycogen levels after up to 4 h of incubation. Lactate from the medium is the source for the observed glycogen synthesis, as the presence of [14C]glycogen after the addition to I-CCM with [14C] lactate was demonstrated. The existence of functional gluconeogenesis in dog sperm was also sustained by the presence of key enzymes of this metabolic pathway, such as fructose 1,6-bisphophatase and aldolase B. On the other hand, glycogen metabolism from gluconeogenic sources was important in the maintenance of a correct in vitro fertilization after incubation in the I-CCM. This was demonstrated after the addition of phenylacetic acid (PAA) to I-CCM. In the presence of PAA, in vitro capacitation of dog spermatozoa suffered alterations, which translated into changes in capacitation functional markers, like the increase in the percentage of altered acrosomes, a distinct motion pattern, decrease or even disappearance of capacitation-induced tyrosine phosphorylation, and increased heterogeneity of the chlorotetracycline pattern in capacitated cells. Thus, this is the first report indicating the existence of a functional glyconeogenesis in mammalian spermatozoa. Moreover, gluconeogenesis-linked glycogen metabolism seems to be of importance in the maintenance of a correct in vitro capacitation in dog sperm in the absence of hexoses in the medium.
Original languageEnglish
Pages (from-to)1437-1445
JournalBiology of Reproduction
Volume71
DOIs
Publication statusPublished - 1 Nov 2004

Keywords

  • Dog sperm
  • Gamete biology
  • Gluconeogenesis
  • Glycogen metabolism
  • In vitro capacitation
  • Sperm
  • Sperm capacitation

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    Albarracín, J. L., Fernández-Novell, J. M., Ballester, J., Rauch, M. C., Quintero-Moreno, A., Peña, A., Mogas, T., Rigau, T., Yañez, A., Guinovart, J. J., Siebe, J. C., Concha, I. I., & Rodriguez Gil, J. E. (2004). Gluconeogenesis-linked glycogen metabolism is important in the achievement of in vitro capacitation of dog spermatozoa in a medium without glucose. Biology of Reproduction, 71, 1437-1445. https://doi.org/10.1095/biolreprod.104.029041