Genotoxicity of copper oxide nanoparticles in Drosophila melanogaster

E.R. Carmona, C. Inostroza-Blancheteau, L. Rubio, R. Marcos

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38 Citations (Scopus)


© 2015 Elsevier B.V. Copper oxide nanoparticles (CuONPs) are used as semiconductors, catalysts, gas sensors, and antimicrobial agents. We have used the comet and wing-spot assays in Drosophila melanogaster to assess the genotoxicity of CuONPs and ionic copper (CuSO<inf>4</inf>). Lipid peroxidation analysis was also performed (Thiobarbituric Acid Assay, TBARS). In larval hemocytes, both CuONPs and CuSO<inf>4</inf> caused significant dose-dependent increases in DNA damage (comet assay). In the wing-spot assay, an increase in the frequency of mutant spots was observed in the wings of the adults; CuONPs were more effective than was CuSO<inf>4</inf>. Both agents induced TBARS; again, CuONPs were more active than was CuSO<inf>4</inf>. The results indicate that CuONPs are genotoxic in Drosophila, and these effects may be mediated by oxidative stress. Most of the effects appear to be related to the presence of copper ions.
Original languageEnglish
Pages (from-to)1-11
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Publication statusPublished - 1 Sept 2015


  • Comet assay
  • DNA damage
  • Hemocytes
  • Malondialdehyde
  • Mitotic recombination
  • Wing-spot test


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