TY - JOUR
T1 - Genotoxicity of copper oxide nanoparticles in Drosophila melanogaster
AU - Carmona, Erico R.
AU - Inostroza-Blancheteau, Claudio
AU - Obando, Veroska
AU - Rubio, Laura
AU - Marcos, Ricard
PY - 2015/9
Y1 - 2015/9
N2 - © 2015 Elsevier B.V. Copper oxide nanoparticles (CuONPs) are used as semiconductors, catalysts, gas sensors, and antimicrobial agents. We have used the comet and wing-spot assays in Drosophila melanogaster to assess the genotoxicity of CuONPs and ionic copper (CuSO4). Lipid peroxidation analysis was also performed (Thiobarbituric Acid Assay, TBARS). In larval hemocytes, both CuONPs and CuSO4 caused significant dose-dependent increases in DNA damage (comet assay). In the wing-spot assay, an increase in the frequency of mutant spots was observed in the wings of the adults; CuONPs were more effective than was CuSO4. Both agents induced TBARS; again, CuONPs were more active than was CuSO4. The results indicate that CuONPs are genotoxic in Drosophila, and these effects may be mediated by oxidative stress. Most of the effects appear to be related to the presence of copper ions.
AB - © 2015 Elsevier B.V. Copper oxide nanoparticles (CuONPs) are used as semiconductors, catalysts, gas sensors, and antimicrobial agents. We have used the comet and wing-spot assays in Drosophila melanogaster to assess the genotoxicity of CuONPs and ionic copper (CuSO4). Lipid peroxidation analysis was also performed (Thiobarbituric Acid Assay, TBARS). In larval hemocytes, both CuONPs and CuSO4 caused significant dose-dependent increases in DNA damage (comet assay). In the wing-spot assay, an increase in the frequency of mutant spots was observed in the wings of the adults; CuONPs were more effective than was CuSO4. Both agents induced TBARS; again, CuONPs were more active than was CuSO4. The results indicate that CuONPs are genotoxic in Drosophila, and these effects may be mediated by oxidative stress. Most of the effects appear to be related to the presence of copper ions.
KW - Comet assay
KW - DNA damage
KW - Hemocytes
KW - Malondialdehyde
KW - Mitotic recombination
KW - Wing-spot test
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=uab_pure&SrcAuth=WosAPI&KeyUT=WOS:000361778300001&DestLinkType=FullRecord&DestApp=WOS_CPL
U2 - 10.1016/j.mrgentox.2015.07.006
DO - 10.1016/j.mrgentox.2015.07.006
M3 - Article
C2 - 26338537
SN - 1383-5718
VL - 791
SP - 1
EP - 11
JO - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
JF - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
ER -