Genotoxic analysis of silver nanoparticles in Drosophila

Eşref Demir, Gerard Vales, Bülent Kaya, Amadeu Creus, Ricardo Marcos

Research output: Contribution to journalArticleResearchpeer-review

90 Citations (Scopus)


Health risk assessment of nanomaterials is an emergent field, genotoxicity being an important endpoint to be tested. Since in vivo studies offer many advantages, such as the study of the bioavailability of nanomaterials to sensitive target cells, we propose Drosophila as a useful model for the study of the toxic and genotoxic risks associated with nanoparticle exposure. In this work we have carried out a genotoxic evaluation of silver nanoparticles in Drosophila by using the wing somatic mutation and recombination test. This test is based on the principle that loss of heterozygosis and the corresponding expression of the suitable recessive markers, multiple wing hairs and flare-3, can lead to the formation of mutant clones in larval cells, which are expressed as mutant spots on the wings of adult flies. Silver nanoparticles were supplied to third instar larvae at concentrations ranging from 0.110 mM. The results showed that small but significant increases in the frequency of total spots were observed, thus indicating that silver nanoparticles were able to induce genotoxic activity in the wing spot assay of D. melanogaster, mainly via the induction of somatic recombination. These positive results obtained with silver nanoparticles contrast with the negative findings obtained when silver nitrate was tested. © 2011 Informa UK, Ltd.
Original languageEnglish
Pages (from-to)417-424
Publication statusPublished - 1 Sep 2011


  • Drosophila melanogaster
  • Genotoxicity
  • Silver nanoparticles
  • Somatic recombination
  • Wing spot assay


Dive into the research topics of 'Genotoxic analysis of silver nanoparticles in Drosophila'. Together they form a unique fingerprint.

Cite this