Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines

Jordi Camps; Itziar Salaverria; Maria J. Garcia; Esther Prat; Sílvia Beà; Jessica C. Pole; Lluis Hernández; Javier Del Rey; Juan Cruz Cigudosa; Marta Bernués; Carlos Caldas; Dolors Colomer; Rosa Miró; Elías Campo

doi:10.1016/j.leukres.2005.11.013

Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines

Jordi Camps, Itziar Salaverria, Maria J. Garcia, Esther Prat, Sílvia Beà, Jessica C. Pole, Lluis Hernández, Javier Del Rey, Juan Cruz Cigudosa, Marta Bernués, Carlos Caldas, Dolors Colomer, Rosa Miró, Elías Campo

Research output: Contribution to journal › Article › Research › peer-review

41 Citations (Scopus)

Abstract

Mantle-cell lymphoma (MCL) is genetically characterized by 11q13 chromosomal translocations involving the CCND1 gene. We have characterized five MCL cell lines, JVM-2, GRANTA-519, REC-1, JEKO-1, and NCEB-1, combining metaphase and array comparative genomic hybridization, multicolor-FISH, and molecular analysis. Our results revealed common gained regions at 2p14, 9q31.2-qter, 11q13.1-q21, 13q14-q21.2, 13q34-qter and 18q21.1-q22.1, and losses at 1p21.2-p31.1, 2p11.2, 8p21.2-pter, 9p21.3-pter, 11q23.3-qter, 17p11.2-pter, and 17q21.2-q22.2. All cell lines except JVM-2, displayed moderate or high numerical chromosome instability. In addition, an ongoing level of chromosome rearrangements was observed in REC-1. Surprisingly, NCEB-1 carried several stable mouse chromosomes and showed expression of both human and murine bcl-2 protein. Our findings indicate that these cell lines represent three patterns of chromosome evolution in MCL and may be useful to understand the pathogenesis of this neoplasm. © 2005 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	923-934
Journal	Leukemia Research
Volume	30
Issue number	8
DOIs	https://doi.org/10.1016/j.leukres.2005.11.013
Publication status	Published - 1 Aug 2006

Keywords

Cell cross-contamination
Cell fusion
Cell lines
Chromosomal instability
Comparative genomic hybridization
Mantle-cell lymphoma
Multicolor-FISH

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Camps, Jordi ; Salaverria, Itziar ; Garcia, Maria J. ; Prat, Esther ; Beà, Sílvia ; Pole, Jessica C. ; Hernández, Lluis ; Rey, Javier Del ; Cigudosa, Juan Cruz ; Bernués, Marta ; Caldas, Carlos ; Colomer, Dolors ; Miró, Rosa ; Campo, Elías. / Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. In: Leukemia Research. 2006 ; Vol. 30, No. 8. pp. 923-934.

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title = "Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines",

abstract = "Mantle-cell lymphoma (MCL) is genetically characterized by 11q13 chromosomal translocations involving the CCND1 gene. We have characterized five MCL cell lines, JVM-2, GRANTA-519, REC-1, JEKO-1, and NCEB-1, combining metaphase and array comparative genomic hybridization, multicolor-FISH, and molecular analysis. Our results revealed common gained regions at 2p14, 9q31.2-qter, 11q13.1-q21, 13q14-q21.2, 13q34-qter and 18q21.1-q22.1, and losses at 1p21.2-p31.1, 2p11.2, 8p21.2-pter, 9p21.3-pter, 11q23.3-qter, 17p11.2-pter, and 17q21.2-q22.2. All cell lines except JVM-2, displayed moderate or high numerical chromosome instability. In addition, an ongoing level of chromosome rearrangements was observed in REC-1. Surprisingly, NCEB-1 carried several stable mouse chromosomes and showed expression of both human and murine bcl-2 protein. Our findings indicate that these cell lines represent three patterns of chromosome evolution in MCL and may be useful to understand the pathogenesis of this neoplasm. {\textcopyright} 2005 Elsevier Ltd. All rights reserved.",

keywords = "Cell cross-contamination, Cell fusion, Cell lines, Chromosomal instability, Comparative genomic hybridization, Mantle-cell lymphoma, Multicolor-FISH",

author = "Jordi Camps and Itziar Salaverria and Garcia, {Maria J.} and Esther Prat and S{\'i}lvia Be{\`a} and Pole, {Jessica C.} and Lluis Hern{\'a}ndez and Rey, {Javier Del} and Cigudosa, {Juan Cruz} and Marta Bernu{\'e}s and Carlos Caldas and Dolors Colomer and Rosa Mir{\'o} and El{\'i}as Campo",

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day = "1",

doi = "10.1016/j.leukres.2005.11.013",

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journal = "Leukemia Research",

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Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. / Camps, Jordi; Salaverria, Itziar; Garcia, Maria J.; Prat, Esther; Beà, Sílvia; Pole, Jessica C.; Hernández, Lluis; Rey, Javier Del; Cigudosa, Juan Cruz; Bernués, Marta; Caldas, Carlos; Colomer, Dolors; Miró, Rosa; Campo, Elías.

In: Leukemia Research, Vol. 30, No. 8, 01.08.2006, p. 923-934.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines

AU - Camps, Jordi

AU - Salaverria, Itziar

AU - Garcia, Maria J.

AU - Prat, Esther

AU - Beà, Sílvia

AU - Pole, Jessica C.

AU - Hernández, Lluis

AU - Rey, Javier Del

AU - Cigudosa, Juan Cruz

AU - Bernués, Marta

AU - Caldas, Carlos

AU - Colomer, Dolors

AU - Miró, Rosa

AU - Campo, Elías

PY - 2006/8/1

Y1 - 2006/8/1

N2 - Mantle-cell lymphoma (MCL) is genetically characterized by 11q13 chromosomal translocations involving the CCND1 gene. We have characterized five MCL cell lines, JVM-2, GRANTA-519, REC-1, JEKO-1, and NCEB-1, combining metaphase and array comparative genomic hybridization, multicolor-FISH, and molecular analysis. Our results revealed common gained regions at 2p14, 9q31.2-qter, 11q13.1-q21, 13q14-q21.2, 13q34-qter and 18q21.1-q22.1, and losses at 1p21.2-p31.1, 2p11.2, 8p21.2-pter, 9p21.3-pter, 11q23.3-qter, 17p11.2-pter, and 17q21.2-q22.2. All cell lines except JVM-2, displayed moderate or high numerical chromosome instability. In addition, an ongoing level of chromosome rearrangements was observed in REC-1. Surprisingly, NCEB-1 carried several stable mouse chromosomes and showed expression of both human and murine bcl-2 protein. Our findings indicate that these cell lines represent three patterns of chromosome evolution in MCL and may be useful to understand the pathogenesis of this neoplasm. © 2005 Elsevier Ltd. All rights reserved.

AB - Mantle-cell lymphoma (MCL) is genetically characterized by 11q13 chromosomal translocations involving the CCND1 gene. We have characterized five MCL cell lines, JVM-2, GRANTA-519, REC-1, JEKO-1, and NCEB-1, combining metaphase and array comparative genomic hybridization, multicolor-FISH, and molecular analysis. Our results revealed common gained regions at 2p14, 9q31.2-qter, 11q13.1-q21, 13q14-q21.2, 13q34-qter and 18q21.1-q22.1, and losses at 1p21.2-p31.1, 2p11.2, 8p21.2-pter, 9p21.3-pter, 11q23.3-qter, 17p11.2-pter, and 17q21.2-q22.2. All cell lines except JVM-2, displayed moderate or high numerical chromosome instability. In addition, an ongoing level of chromosome rearrangements was observed in REC-1. Surprisingly, NCEB-1 carried several stable mouse chromosomes and showed expression of both human and murine bcl-2 protein. Our findings indicate that these cell lines represent three patterns of chromosome evolution in MCL and may be useful to understand the pathogenesis of this neoplasm. © 2005 Elsevier Ltd. All rights reserved.

KW - Cell cross-contamination

KW - Cell fusion

KW - Cell lines

KW - Chromosomal instability

KW - Comparative genomic hybridization

KW - Mantle-cell lymphoma

KW - Multicolor-FISH

U2 - 10.1016/j.leukres.2005.11.013

DO - 10.1016/j.leukres.2005.11.013

M3 - Article

VL - 30

SP - 923

EP - 934

JO - Leukemia Research

JF - Leukemia Research

SN - 0145-2126

IS - 8

ER -