Genomic analysis of different Chlorobium strains by pulsed-field gel electrophoresis and ribotyping

Sebastián Méndez-Alvarez; Isabel Esteve; Ricardo Guerrero; Núria Gaju

doi:10.1099/00207713-46-4-1177

Genomic analysis of different Chlorobium strains by pulsed-field gel electrophoresis and ribotyping

Sebastián Méndez-Alvarez, Isabel Esteve, Ricardo Guerrero, Núria Gaju

Research output: Contribution to journal › Article › Research › peer-review

4 Citations (Scopus)

Abstract

Pulsed-field gel electrophoresis was used to characterize the genomes of 15 Chlorobium strains. An analysis of chromosomal macrorestriction patterns allowed us to determine the chromosome sizes, which ranged from 1,435 to 3,342 kb depending on the strain. Moreover, this analysis revealed that, even though the genus Chlorobium is a phylogenetically coherent taxon, there is a great deal of genomic heterogeneity within it. This heterogeneity was corroborated by performing a ribotyping analysis, which showed that the rRNA hybridization patterns of most strains are unique.

Original language	English
Pages (from-to)	1177-1179
Journal	International Journal of Systematic Bacteriology
Volume	46
Issue number	4
DOIs	https://doi.org/10.1099/00207713-46-4-1177
Publication status	Published - 1 Jan 1996

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abstract = "Pulsed-field gel electrophoresis was used to characterize the genomes of 15 Chlorobium strains. An analysis of chromosomal macrorestriction patterns allowed us to determine the chromosome sizes, which ranged from 1,435 to 3,342 kb depending on the strain. Moreover, this analysis revealed that, even though the genus Chlorobium is a phylogenetically coherent taxon, there is a great deal of genomic heterogeneity within it. This heterogeneity was corroborated by performing a ribotyping analysis, which showed that the rRNA hybridization patterns of most strains are unique.",

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AB - Pulsed-field gel electrophoresis was used to characterize the genomes of 15 Chlorobium strains. An analysis of chromosomal macrorestriction patterns allowed us to determine the chromosome sizes, which ranged from 1,435 to 3,342 kb depending on the strain. Moreover, this analysis revealed that, even though the genus Chlorobium is a phylogenetically coherent taxon, there is a great deal of genomic heterogeneity within it. This heterogeneity was corroborated by performing a ribotyping analysis, which showed that the rRNA hybridization patterns of most strains are unique.

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