Genomic analysis identifies association of Fusobacterium with colorectal carcinoma

Aleksandar D. Kostic, Dirk Gevers, Chandra Sekhar Pedamallu, Monia Michaud, Fujiko Duke, Ashlee M. Earl, Akinyemi I. Ojesina, Joonil Jung, Adam J. Bass, Josep Tabernero, José Baselga, Chen Liu, Ramesh A. Shivdasani, Shuji Ogino, Bruce W. Birren, Curtis Huttenhower, Wendy S. Garrett, Matthew Meyerson

Research output: Contribution to journalArticleResearchpeer-review

906 Citations (Scopus)

Abstract

The tumor microenvironment of colorectal carcinoma is a complex community of genomically altered cancer cells, nonneoplastic cells, and a diverse collection of microorganisms. Each of these components may contribute to carcinogenesis; however, the role of the microbiota is the least well understood. We have characterized the composition of the microbiota in colorectal carcinoma using whole genome sequences from nine tumor/normal pairs. Fusobacterium sequences were enriched in carcinomas, confirmed by quantitative PCR and 16S rDNA sequence analysis of 95 carcinoma/normal DNA pairs, while the Bacteroidetes and Firmicutes phyla were depleted in tumors. Fusobacteria were also visualized within colorectal tumors using FISH. These findings reveal alterations in the colorectal cancer microbiota; however, the precise role of Fusobacteria in colorectal carcinoma pathogenesis requires further investigation. © 2012 by Cold Spring Harbor Laboratory Press.
Original languageEnglish
Pages (from-to)292-298
JournalGenome Research
Volume22
Issue number2
DOIs
Publication statusPublished - 1 Feb 2012

Fingerprint Dive into the research topics of 'Genomic analysis identifies association of Fusobacterium with colorectal carcinoma'. Together they form a unique fingerprint.

Cite this