Genomic analysis identifies association of Fusobacterium with colorectal carcinoma

Aleksandar D. Kostic; Dirk Gevers; Chandra Sekhar Pedamallu; Monia Michaud; Fujiko Duke; Ashlee M. Earl; Akinyemi I. Ojesina; Joonil Jung; Adam J. Bass; Josep Tabernero; José Baselga; Chen Liu; Ramesh A. Shivdasani; Shuji Ogino; Bruce W. Birren; Curtis Huttenhower; Wendy S. Garrett; Matthew Meyerson

doi:10.1101/gr.126573.111

Genomic analysis identifies association of Fusobacterium with colorectal carcinoma

Aleksandar D. Kostic, Dirk Gevers, Chandra Sekhar Pedamallu, Monia Michaud, Fujiko Duke, Ashlee M. Earl, Akinyemi I. Ojesina, Joonil Jung, Adam J. Bass, Josep Tabernero, José Baselga, Chen Liu, Ramesh A. Shivdasani, Shuji Ogino, Bruce W. Birren, Curtis Huttenhower, Wendy S. Garrett, Matthew Meyerson

Department of Medicine

Research output: Contribution to journal › Article › Research › peer-review

1225 Citations (Scopus)

Abstract

The tumor microenvironment of colorectal carcinoma is a complex community of genomically altered cancer cells, nonneoplastic cells, and a diverse collection of microorganisms. Each of these components may contribute to carcinogenesis; however, the role of the microbiota is the least well understood. We have characterized the composition of the microbiota in colorectal carcinoma using whole genome sequences from nine tumor/normal pairs. Fusobacterium sequences were enriched in carcinomas, confirmed by quantitative PCR and 16S rDNA sequence analysis of 95 carcinoma/normal DNA pairs, while the Bacteroidetes and Firmicutes phyla were depleted in tumors. Fusobacteria were also visualized within colorectal tumors using FISH. These findings reveal alterations in the colorectal cancer microbiota; however, the precise role of Fusobacteria in colorectal carcinoma pathogenesis requires further investigation. © 2012 by Cold Spring Harbor Laboratory Press.

Original language	English
Pages (from-to)	292-298
Journal	Genome Research
Volume	22
Issue number	2
DOIs	https://doi.org/10.1101/gr.126573.111
Publication status	Published - 1 Feb 2012

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Kostic, A. D., Gevers, D., Pedamallu, C. S., Michaud, M., Duke, F., Earl, A. M., Ojesina, A. I., Jung, J., Bass, A. J., Tabernero, J., Baselga, J., Liu, C., Shivdasani, R. A., Ogino, S., Birren, B. W., Huttenhower, C., Garrett, W. S., & Meyerson, M. (2012). Genomic analysis identifies association of Fusobacterium with colorectal carcinoma. Genome Research, 22(2), 292-298. https://doi.org/10.1101/gr.126573.111

@article{48c42f74e2ed4449af2da163b3680f6a,

title = "Genomic analysis identifies association of Fusobacterium with colorectal carcinoma",

abstract = "The tumor microenvironment of colorectal carcinoma is a complex community of genomically altered cancer cells, nonneoplastic cells, and a diverse collection of microorganisms. Each of these components may contribute to carcinogenesis; however, the role of the microbiota is the least well understood. We have characterized the composition of the microbiota in colorectal carcinoma using whole genome sequences from nine tumor/normal pairs. Fusobacterium sequences were enriched in carcinomas, confirmed by quantitative PCR and 16S rDNA sequence analysis of 95 carcinoma/normal DNA pairs, while the Bacteroidetes and Firmicutes phyla were depleted in tumors. Fusobacteria were also visualized within colorectal tumors using FISH. These findings reveal alterations in the colorectal cancer microbiota; however, the precise role of Fusobacteria in colorectal carcinoma pathogenesis requires further investigation. {\textcopyright} 2012 by Cold Spring Harbor Laboratory Press.",

author = "Kostic, {Aleksandar D.} and Dirk Gevers and Pedamallu, {Chandra Sekhar} and Monia Michaud and Fujiko Duke and Earl, {Ashlee M.} and Ojesina, {Akinyemi I.} and Joonil Jung and Bass, {Adam J.} and Josep Tabernero and Jos{\'e} Baselga and Chen Liu and Shivdasani, {Ramesh A.} and Shuji Ogino and Birren, {Bruce W.} and Curtis Huttenhower and Garrett, {Wendy S.} and Matthew Meyerson",

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doi = "10.1101/gr.126573.111",

language = "English",

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Kostic, AD, Gevers, D, Pedamallu, CS, Michaud, M, Duke, F, Earl, AM, Ojesina, AI, Jung, J, Bass, AJ, Tabernero, J, Baselga, J, Liu, C, Shivdasani, RA, Ogino, S, Birren, BW, Huttenhower, C, Garrett, WS & Meyerson, M 2012, 'Genomic analysis identifies association of Fusobacterium with colorectal carcinoma', Genome Research, vol. 22, no. 2, pp. 292-298. https://doi.org/10.1101/gr.126573.111

TY - JOUR

T1 - Genomic analysis identifies association of Fusobacterium with colorectal carcinoma

AU - Kostic, Aleksandar D.

AU - Gevers, Dirk

AU - Pedamallu, Chandra Sekhar

AU - Michaud, Monia

AU - Duke, Fujiko

AU - Earl, Ashlee M.

AU - Ojesina, Akinyemi I.

AU - Jung, Joonil

AU - Bass, Adam J.

AU - Tabernero, Josep

AU - Baselga, José

AU - Liu, Chen

AU - Shivdasani, Ramesh A.

AU - Ogino, Shuji

AU - Birren, Bruce W.

AU - Huttenhower, Curtis

AU - Garrett, Wendy S.

AU - Meyerson, Matthew

PY - 2012/2/1

Y1 - 2012/2/1

N2 - The tumor microenvironment of colorectal carcinoma is a complex community of genomically altered cancer cells, nonneoplastic cells, and a diverse collection of microorganisms. Each of these components may contribute to carcinogenesis; however, the role of the microbiota is the least well understood. We have characterized the composition of the microbiota in colorectal carcinoma using whole genome sequences from nine tumor/normal pairs. Fusobacterium sequences were enriched in carcinomas, confirmed by quantitative PCR and 16S rDNA sequence analysis of 95 carcinoma/normal DNA pairs, while the Bacteroidetes and Firmicutes phyla were depleted in tumors. Fusobacteria were also visualized within colorectal tumors using FISH. These findings reveal alterations in the colorectal cancer microbiota; however, the precise role of Fusobacteria in colorectal carcinoma pathogenesis requires further investigation. © 2012 by Cold Spring Harbor Laboratory Press.

AB - The tumor microenvironment of colorectal carcinoma is a complex community of genomically altered cancer cells, nonneoplastic cells, and a diverse collection of microorganisms. Each of these components may contribute to carcinogenesis; however, the role of the microbiota is the least well understood. We have characterized the composition of the microbiota in colorectal carcinoma using whole genome sequences from nine tumor/normal pairs. Fusobacterium sequences were enriched in carcinomas, confirmed by quantitative PCR and 16S rDNA sequence analysis of 95 carcinoma/normal DNA pairs, while the Bacteroidetes and Firmicutes phyla were depleted in tumors. Fusobacteria were also visualized within colorectal tumors using FISH. These findings reveal alterations in the colorectal cancer microbiota; however, the precise role of Fusobacteria in colorectal carcinoma pathogenesis requires further investigation. © 2012 by Cold Spring Harbor Laboratory Press.

U2 - 10.1101/gr.126573.111

DO - 10.1101/gr.126573.111

M3 - Article

SN - 1088-9051

VL - 22

SP - 292

EP - 298

JO - Genome Research

JF - Genome Research

IS - 2

ER -

Genomic analysis identifies association of Fusobacterium with colorectal carcinoma

Abstract

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