Genome-scale metabolic reconstruction for the insidious bacterium in aquaculture Piscirickettsia salmonis

Pablo Fuentealba, Camila Aros, Yesenia Latorre, Irene Martínez, Sergio Marshall, Pau Ferrer, Joan Albiol, Claudia Altamirano

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Abstract

© 2016 Elsevier Ltd Piscirickettsia salmonis is a fish bacterium that causes the disease piscirickettsiosis in salmonids. This pathology is partially controlled by vaccines. The lack of knowledge has hindered its culture on laboratory and industrial scale. The study describes the metabolic phenotype of P. salmonis in culture. This study presents the first genome-scale model (iPF215) of the LF-89 strain of P. salmonis, describing the central metabolic pathway, biosynthesis and molecule degradation and transport mechanisms. The model was adjusted with experiment data, allowing the identification of the capacities that were not predicted by the automatic annotation of the genome sequences. The iPF215 model is comprised of 417 metabolites, 445 reactions and 215 genes, was used to reproduce the growth of P. salmonis (μmax 0.052 ± 0.005 h−1). The metabolic reconstruction of the P. salmonis LF-89 strain obtained in this research provides a baseline that describes the metabolic capacities of the bacterium and is the basis for developing improvements to its cultivation for vaccine formulation.
Original languageEnglish
Pages (from-to)105-114
JournalBioresource Technology
Volume223
DOIs
Publication statusPublished - 1 Jan 2017

Keywords

  • Defined medium
  • Genome-scale metabolic model
  • Metabolic reconstruction
  • Nutritional requirement
  • Piscirickettsia salmonis

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