Genome-scale metabolic model of methylotrophic yeast Pichia pastoris and its use for in silico analysis of heterologous protein production

Seung Bum Sohn, Alexandra B. Graf, Tae Yong Kim, Brigitte Gasser, Michael Maurer, Pau Ferrer, Diethard Mattanovich, Sang Yup Lee

    Research output: Contribution to journalArticleResearchpeer-review

    85 Citations (Scopus)

    Abstract

    The methylotrophic yeast Pichia pastoris has gained much attention during the last decade as a platform for producing heterologous recombinant proteins of pharmaceutical importance, due to its ability to reproduce post-translational modification similar to higher eukaryotes. With the recent release of the full genome sequence for P. pastoris, in-depth study of its functions has become feasible. Here we present the first reconstruction of the genome-scale metabolic model of the eukaryote P. pastoris type strain DSMZ 70382, PpaMBEL1254, consisting of 1254 metabolic reactions and 1147 metabolites compartmentalized into eight different regions to represent organelles. Additionally, equations describing the production of two heterologous proteins, human serum albumin and human superoxide dismutase, were incorporated. The protein-producing model versions of PpaMBEL1254 were then analyzed to examine the impact on oxygen limitation on protein production. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.
    Original languageEnglish
    Pages (from-to)705-715
    JournalBiotechnology Journal
    Volume5
    Issue number7
    DOIs
    Publication statusPublished - 1 Jan 2010

    Keywords

    • Genome-scale metabolic model
    • Heterologous protein production
    • HSA
    • Hsod
    • Pichia pastoris

    Fingerprint

    Dive into the research topics of 'Genome-scale metabolic model of methylotrophic yeast Pichia pastoris and its use for in silico analysis of heterologous protein production'. Together they form a unique fingerprint.

    Cite this