Genetic diversity of rhinoviruses detected at a tertiary hospital in Catalonia (Spain) during the 2014-2017 seasons

Cristina Andrés; Paula Peremiquel-Trillas; Laura Gimferrer; Anna Isern; Maria Piñana; José Ángel Rodrigo-Pendás; Maria Gema Codina; María Del Carmen Martín; Francisco Fuentes; Susana Rubio; Magda Campins-Martí; Tomàs Pumarola; Andrés Antón

doi:10.2217/fmb-2018-0151

Genetic diversity of rhinoviruses detected at a tertiary hospital in Catalonia (Spain) during the 2014-2017 seasons

Cristina Andrés, Paula Peremiquel-Trillas, Laura Gimferrer, Anna Isern, Maria Piñana, José Ángel Rodrigo-Pendás, Maria Gema Codina, María Del Carmen Martín, Francisco Fuentes, Susana Rubio, Magda Campins-Martí, Tomàs Pumarola, Andrés Antón

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4 Citations (Scopus)

Abstract

© 2018 Future Medicine Ltd. Aim: To describe the genetic diversity of rhinovirus (RV) from patients attended at a tertiary hospital in Barcelona (Spain) from October 2014 to May 2017. Methods: RV detection was performed by real-time multiplex RT-PCR. A specific real-time quantitive retrotranscription PCR (qRT-PCR) was carried out to select those samples (Ct < 35) for molecular characterization based on partial VP4/2 protein. Results: Phylogenetic characterization revealed proportions of 63% RV-A, 6% RV-B and 31% RV-C (119 different types). RV-A circulated throughout all the study period, with a minor circulation during winter, just when RV-C prevailed. Differences between age medians by RV-specie were reported. Conclusion: The large genetic diversity of RV detected in our area is described here. The variable cocirculation of multiple RV types is also reported, showing differences by age.

Original language	English
Pages (from-to)	1565-1573
Journal	Future Microbiology
Volume	13
DOIs	https://doi.org/10.2217/fmb-2018-0151
Publication status	Published - 1 Nov 2018

Keywords

evolution
genetic diversity
mild respiratory diseases
molecular epidemiology
pediatric patients
respiratory tract infection
respiratory virus
rhinovirus
RV circulation
surveillance

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10.2217/fmb-2018-0151

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Andrés, C., Peremiquel-Trillas, P., Gimferrer, L., Isern, A., Piñana, M., Rodrigo-Pendás, J. Á., Codina, M. G., Martín, M. D. C., Fuentes, F., Rubio, S., Campins-Martí, M., Pumarola, T., & Antón, A. (2018). Genetic diversity of rhinoviruses detected at a tertiary hospital in Catalonia (Spain) during the 2014-2017 seasons. Future Microbiology, 13, 1565-1573. https://doi.org/10.2217/fmb-2018-0151

@article{13cea978398f423dbeb0f6303a03d338,

title = "Genetic diversity of rhinoviruses detected at a tertiary hospital in Catalonia (Spain) during the 2014-2017 seasons",

abstract = "{\textcopyright} 2018 Future Medicine Ltd. Aim: To describe the genetic diversity of rhinovirus (RV) from patients attended at a tertiary hospital in Barcelona (Spain) from October 2014 to May 2017. Methods: RV detection was performed by real-time multiplex RT-PCR. A specific real-time quantitive retrotranscription PCR (qRT-PCR) was carried out to select those samples (Ct < 35) for molecular characterization based on partial VP4/2 protein. Results: Phylogenetic characterization revealed proportions of 63% RV-A, 6% RV-B and 31% RV-C (119 different types). RV-A circulated throughout all the study period, with a minor circulation during winter, just when RV-C prevailed. Differences between age medians by RV-specie were reported. Conclusion: The large genetic diversity of RV detected in our area is described here. The variable cocirculation of multiple RV types is also reported, showing differences by age.",

keywords = "evolution, genetic diversity, mild respiratory diseases, molecular epidemiology, pediatric patients, respiratory tract infection, respiratory virus, rhinovirus, RV circulation, surveillance",

author = "Cristina Andr{\'e}s and Paula Peremiquel-Trillas and Laura Gimferrer and Anna Isern and Maria Pi{\~n}ana and Rodrigo-Pend{\'a}s, {Jos{\'e} {\'A}ngel} and Codina, {Maria Gema} and Mart{\'i}n, {Mar{\'i}a Del Carmen} and Francisco Fuentes and Susana Rubio and Magda Campins-Mart{\'i} and Tom{\`a}s Pumarola and Andr{\'e}s Ant{\'o}n",

year = "2018",

month = nov,

day = "1",

doi = "10.2217/fmb-2018-0151",

language = "English",

volume = "13",

pages = "1565--1573",

journal = "Future Microbiology",

issn = "1746-0913",

publisher = "Future Medicine Ltd.",

}

Andrés, C, Peremiquel-Trillas, P, Gimferrer, L, Isern, A, Piñana, M, Rodrigo-Pendás, JÁ, Codina, MG, Martín, MDC, Fuentes, F, Rubio, S, Campins-Martí, M, Pumarola, T & Antón, A 2018, 'Genetic diversity of rhinoviruses detected at a tertiary hospital in Catalonia (Spain) during the 2014-2017 seasons', Future Microbiology, vol. 13, pp. 1565-1573. https://doi.org/10.2217/fmb-2018-0151

TY - JOUR

T1 - Genetic diversity of rhinoviruses detected at a tertiary hospital in Catalonia (Spain) during the 2014-2017 seasons

AU - Andrés, Cristina

AU - Peremiquel-Trillas, Paula

AU - Gimferrer, Laura

AU - Isern, Anna

AU - Piñana, Maria

AU - Rodrigo-Pendás, José Ángel

AU - Codina, Maria Gema

AU - Martín, María Del Carmen

AU - Fuentes, Francisco

AU - Rubio, Susana

AU - Campins-Martí, Magda

AU - Pumarola, Tomàs

AU - Antón, Andrés

PY - 2018/11/1

Y1 - 2018/11/1

N2 - © 2018 Future Medicine Ltd. Aim: To describe the genetic diversity of rhinovirus (RV) from patients attended at a tertiary hospital in Barcelona (Spain) from October 2014 to May 2017. Methods: RV detection was performed by real-time multiplex RT-PCR. A specific real-time quantitive retrotranscription PCR (qRT-PCR) was carried out to select those samples (Ct < 35) for molecular characterization based on partial VP4/2 protein. Results: Phylogenetic characterization revealed proportions of 63% RV-A, 6% RV-B and 31% RV-C (119 different types). RV-A circulated throughout all the study period, with a minor circulation during winter, just when RV-C prevailed. Differences between age medians by RV-specie were reported. Conclusion: The large genetic diversity of RV detected in our area is described here. The variable cocirculation of multiple RV types is also reported, showing differences by age.

AB - © 2018 Future Medicine Ltd. Aim: To describe the genetic diversity of rhinovirus (RV) from patients attended at a tertiary hospital in Barcelona (Spain) from October 2014 to May 2017. Methods: RV detection was performed by real-time multiplex RT-PCR. A specific real-time quantitive retrotranscription PCR (qRT-PCR) was carried out to select those samples (Ct < 35) for molecular characterization based on partial VP4/2 protein. Results: Phylogenetic characterization revealed proportions of 63% RV-A, 6% RV-B and 31% RV-C (119 different types). RV-A circulated throughout all the study period, with a minor circulation during winter, just when RV-C prevailed. Differences between age medians by RV-specie were reported. Conclusion: The large genetic diversity of RV detected in our area is described here. The variable cocirculation of multiple RV types is also reported, showing differences by age.

KW - evolution

KW - genetic diversity

KW - mild respiratory diseases

KW - molecular epidemiology

KW - pediatric patients

KW - respiratory tract infection

KW - respiratory virus

KW - rhinovirus

KW - RV circulation

KW - surveillance

U2 - 10.2217/fmb-2018-0151

DO - 10.2217/fmb-2018-0151

M3 - Article

C2 - 30417657

SN - 1746-0913

VL - 13

SP - 1565

EP - 1573

JO - Future Microbiology

JF - Future Microbiology

ER -

Genetic diversity of rhinoviruses detected at a tertiary hospital in Catalonia (Spain) during the 2014-2017 seasons

Abstract

Keywords

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