Genetic and kinetic characterization of the novel AmpC β-lactamases DHA-6 and DHA-7

Francisco José Pérez-Llarena, Laura Zamorano, Frédéric Kerff, Alejandro Beceiro, Patricia García, Elisenda Miró, Nieves Larrosa, Frederic Gómez-Bertomeu, José Antonio Méndez, Juan José González-López, Antonio Oliver, Moreno Galleni, Ferran Navarro, Germán Bou

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4 Citations (Scopus)

Abstract

Copyright © 2014, American Society for Microbiology. All Rights Reserved. During a Spanish surveillance study, two natural variants of DHA β-lactamases, DHA-6 and DHA-7, were found, with the replacements Ala226Thr and Phe322Ser, respectively, with respect to DHA-1. The DHA-6 and DHA-7 enzymes were isolated from Escherichia coli and Enterobacter cloacae clinical isolates, respectively. The aim of this study was to genetically, microbiologically, and biochemically characterize the DHA-6 and DHA-7 β-lactamases. The bla<inf>DHA-6</inf> and bla<inf>DHA-7</inf> genes were located in the I1 and HI2 incompatibility group plasmids of 87.3 and 310.4 kb, respectively. The genetic contexts of bla<inf>DHA-6</inf> and bla<inf>DHA-7</inf> were similar to that already described for the bla<inf>DHA-1</inf> gene and included the qnrB4 and aadA genes. The MICs for cephalothin, aztreonam, cefotaxime, and ceftazidime were 8- to 32-fold lower for DHA-6 than for DHA-1 or DHA-7 expressed in the same isogenic E. coli TG1 strain. Interestingly, the MIC for cefoxitin was higher in the DHA-6-expressing transformant than in DHA-1 or DHA-7. Biochemical studies with pure β-lactamases revealed slightly lower catalytic efficiencies of DHA-6 against cephalothin, ceftazidime, and cefotaxime than those of DHA-1 and DHA-7. To understand this behavior, stability experiments were carried out and showed that the DHA-6 protein displayed significantly higher stability than the DHA-1 and DHA-7 enzymes. The proximity of Thr226 to the N terminus in the tertiary protein structure in DHA-6 may promote this stabilization and, consequently, may induce a slight reduction in the dynamic of this enzyme that primarily affects the hydrolysis of some of the bulkiest antibiotics.
Original languageEnglish
Pages (from-to)6544-6549
JournalAntimicrobial Agents and Chemotherapy
Volume58
Issue number11
DOIs
Publication statusPublished - 1 Nov 2014

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    Pérez-Llarena, F. J., Zamorano, L., Kerff, F., Beceiro, A., García, P., Miró, E., Larrosa, N., Gómez-Bertomeu, F., Méndez, J. A., González-López, J. J., Oliver, A., Galleni, M., Navarro, F., & Bou, G. (2014). Genetic and kinetic characterization of the novel AmpC β-lactamases DHA-6 and DHA-7. Antimicrobial Agents and Chemotherapy, 58(11), 6544-6549. https://doi.org/10.1128/AAC.03144-14