Gene expression profiling in hearts of diabetic mice uncovers a potential role of estrogen-related receptor γ in diabetic cardiomyopathy

Jaime Lasheras; Maria Vilà; Mònica Zamora; Efrén Riu; Rosario Pardo; Marcos Poncelas; Ildefonso Cases; Marisol Ruiz-Meana; Cristina Hernández; Juan E. Feliu; Rafael Simó; David García-Dorado; Josep A. Villena

doi:10.1016/j.mce.2016.04.004

Gene expression profiling in hearts of diabetic mice uncovers a potential role of estrogen-related receptor γ in diabetic cardiomyopathy

Jaime Lasheras, Maria Vilà, Mònica Zamora, Efrén Riu, Rosario Pardo, Marcos Poncelas, Ildefonso Cases, Marisol Ruiz-Meana, Cristina Hernández, Juan E. Feliu, Rafael Simó, David García-Dorado, Josep A. Villena

Department of Medicine

Research output: Contribution to journal › Article › Research › peer-review

9 Citations (Scopus)

Abstract

© 2016 Elsevier Ireland Ltd Diabetic cardiomyopathy is characterized by an abnormal oxidative metabolism, but the underlying mechanisms remain to be defined. To uncover potential mechanisms involved in the pathophysiology of diabetic cardiomyopathy, we performed a gene expression profiling study in hearts of diabetic db/db mice. Diabetic hearts showed a gene expression pattern characterized by the up-regulation of genes involved in lipid oxidation, together with an abnormal expression of genes related to the cardiac contractile function. A screening for potential regulators of the genes differentially expressed in diabetic mice found that estrogen-related receptor γ (ERRγ) was increased in heart of db/db mice. Overexpression of ERRγ in cultured cardiomyocytes was sufficient to promote the expression of genes involved in lipid oxidation, increase palmitate oxidation and induce cardiomyocyte hypertrophy. Our findings strongly support a role for ERRγ in the metabolic alterations that underlie the development of diabetic cardiomyopathy.

Original language	English
Pages (from-to)	77-88
Journal	Molecular and Cellular Endocrinology
Volume	430
DOIs	https://doi.org/10.1016/j.mce.2016.04.004
Publication status	Published - 15 Jul 2016

Keywords

Diabetes
Estrogen-related receptors
Fatty acid oxidation
Lipid metabolism
Orphan nuclear receptor
Transcriptional regulation of gene expression

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.mce.2016.04.004

Cite this

Lasheras, J., Vilà, M., Zamora, M., Riu, E., Pardo, R., Poncelas, M., Cases, I., Ruiz-Meana, M., Hernández, C., Feliu, J. E., Simó, R., García-Dorado, D., & Villena, J. A. (2016). Gene expression profiling in hearts of diabetic mice uncovers a potential role of estrogen-related receptor γ in diabetic cardiomyopathy. Molecular and Cellular Endocrinology, 430, 77-88. https://doi.org/10.1016/j.mce.2016.04.004

@article{f43cd385c2654a218f8d9597ef7d9ff6,

title = "Gene expression profiling in hearts of diabetic mice uncovers a potential role of estrogen-related receptor γ in diabetic cardiomyopathy",

abstract = "{\textcopyright} 2016 Elsevier Ireland Ltd Diabetic cardiomyopathy is characterized by an abnormal oxidative metabolism, but the underlying mechanisms remain to be defined. To uncover potential mechanisms involved in the pathophysiology of diabetic cardiomyopathy, we performed a gene expression profiling study in hearts of diabetic db/db mice. Diabetic hearts showed a gene expression pattern characterized by the up-regulation of genes involved in lipid oxidation, together with an abnormal expression of genes related to the cardiac contractile function. A screening for potential regulators of the genes differentially expressed in diabetic mice found that estrogen-related receptor γ (ERRγ) was increased in heart of db/db mice. Overexpression of ERRγ in cultured cardiomyocytes was sufficient to promote the expression of genes involved in lipid oxidation, increase palmitate oxidation and induce cardiomyocyte hypertrophy. Our findings strongly support a role for ERRγ in the metabolic alterations that underlie the development of diabetic cardiomyopathy.",

keywords = "Diabetes, Estrogen-related receptors, Fatty acid oxidation, Lipid metabolism, Orphan nuclear receptor, Transcriptional regulation of gene expression",

author = "Jaime Lasheras and Maria Vil{\`a} and M{\`o}nica Zamora and Efr{\'e}n Riu and Rosario Pardo and Marcos Poncelas and Ildefonso Cases and Marisol Ruiz-Meana and Cristina Hern{\'a}ndez and Feliu, {Juan E.} and Rafael Sim{\'o} and David Garc{\'i}a-Dorado and Villena, {Josep A.}",

year = "2016",

month = jul,

day = "15",

doi = "10.1016/j.mce.2016.04.004",

language = "English",

volume = "430",

pages = "77--88",

journal = "Molecular and Cellular Endocrinology",

issn = "0303-7207",

publisher = "Elsevier Ireland Ltd",

}

Lasheras, J, Vilà, M, Zamora, M, Riu, E, Pardo, R, Poncelas, M, Cases, I, Ruiz-Meana, M, Hernández, C, Feliu, JE, Simó, R, García-Dorado, D & Villena, JA 2016, 'Gene expression profiling in hearts of diabetic mice uncovers a potential role of estrogen-related receptor γ in diabetic cardiomyopathy', Molecular and Cellular Endocrinology, vol. 430, pp. 77-88. https://doi.org/10.1016/j.mce.2016.04.004

TY - JOUR

T1 - Gene expression profiling in hearts of diabetic mice uncovers a potential role of estrogen-related receptor γ in diabetic cardiomyopathy

AU - Lasheras, Jaime

AU - Vilà, Maria

AU - Zamora, Mònica

AU - Riu, Efrén

AU - Pardo, Rosario

AU - Poncelas, Marcos

AU - Cases, Ildefonso

AU - Ruiz-Meana, Marisol

AU - Hernández, Cristina

AU - Feliu, Juan E.

AU - Simó, Rafael

AU - García-Dorado, David

AU - Villena, Josep A.

PY - 2016/7/15

Y1 - 2016/7/15

N2 - © 2016 Elsevier Ireland Ltd Diabetic cardiomyopathy is characterized by an abnormal oxidative metabolism, but the underlying mechanisms remain to be defined. To uncover potential mechanisms involved in the pathophysiology of diabetic cardiomyopathy, we performed a gene expression profiling study in hearts of diabetic db/db mice. Diabetic hearts showed a gene expression pattern characterized by the up-regulation of genes involved in lipid oxidation, together with an abnormal expression of genes related to the cardiac contractile function. A screening for potential regulators of the genes differentially expressed in diabetic mice found that estrogen-related receptor γ (ERRγ) was increased in heart of db/db mice. Overexpression of ERRγ in cultured cardiomyocytes was sufficient to promote the expression of genes involved in lipid oxidation, increase palmitate oxidation and induce cardiomyocyte hypertrophy. Our findings strongly support a role for ERRγ in the metabolic alterations that underlie the development of diabetic cardiomyopathy.

AB - © 2016 Elsevier Ireland Ltd Diabetic cardiomyopathy is characterized by an abnormal oxidative metabolism, but the underlying mechanisms remain to be defined. To uncover potential mechanisms involved in the pathophysiology of diabetic cardiomyopathy, we performed a gene expression profiling study in hearts of diabetic db/db mice. Diabetic hearts showed a gene expression pattern characterized by the up-regulation of genes involved in lipid oxidation, together with an abnormal expression of genes related to the cardiac contractile function. A screening for potential regulators of the genes differentially expressed in diabetic mice found that estrogen-related receptor γ (ERRγ) was increased in heart of db/db mice. Overexpression of ERRγ in cultured cardiomyocytes was sufficient to promote the expression of genes involved in lipid oxidation, increase palmitate oxidation and induce cardiomyocyte hypertrophy. Our findings strongly support a role for ERRγ in the metabolic alterations that underlie the development of diabetic cardiomyopathy.

KW - Diabetes

KW - Estrogen-related receptors

KW - Fatty acid oxidation

KW - Lipid metabolism

KW - Orphan nuclear receptor

KW - Transcriptional regulation of gene expression

U2 - 10.1016/j.mce.2016.04.004

DO - 10.1016/j.mce.2016.04.004

M3 - Article

SN - 0303-7207

VL - 430

SP - 77

EP - 88

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

ER -

Gene expression profiling in hearts of diabetic mice uncovers a potential role of estrogen-related receptor γ in diabetic cardiomyopathy

Abstract

Keywords

UN SDGs

Access to Document

Fingerprint

Cite this