Fusion polypeptides in gene cloning: Potential problems due to conformational alterations at the junction

E. Querol, A. Parrilla

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)

Abstract

Many eukaryotic genes are cloned in bacterial hosts as fusion polypeptides. Prediction of the secondary structures for some common prokaryotic fusion polypeptides shows that many junction sites correspond to important secondary structures. It is suggested that such structures could affect (hinder, etc.) the conformation or drive the folding of the neighboring eukaryotic counterparts. Thus the prokaryotic junction should be better performed, in random coil regions, or short fusion prokaryotic polypeptides should be sued. © 1987 Birkhäuser Verlag.
Original languageEnglish
Pages (from-to)920-922
JournalExperientia
Volume43
Issue number8
DOIs
Publication statusPublished - 1 Aug 1987

Keywords

  • fusion polypeptides
  • Molecular cloning
  • protein conformation
  • protein folding

Fingerprint Dive into the research topics of 'Fusion polypeptides in gene cloning: Potential problems due to conformational alterations at the junction'. Together they form a unique fingerprint.

Cite this