Functionalization of 3D scaffolds with protein-releasing biomaterials for intracellular delivery

Joaquin Seras-Franzoso, Christoph Steurer, Mònica Roldán, Meritxell Vendrell, Carla Vidaurre-Agut, Anna Tarruella, Laura Saldaña, Nuria Vilaboa, Marc Parera, Elisa Elizondo, Imma Ratera, Nora Ventosa, Jaume Veciana, Alberto J. Campillo-Fernández, Elena García-Fruitós, Esther Vázquez, Antonio Villaverde

Research output: Contribution to journalArticleResearchpeer-review

21 Citations (Scopus)


Appropriate combinations of mechanical and biological stimuli are required to promote proper colonization of substrate materials in regenerative medicine. In this context, 3D scaffolds formed by compatible and biodegradable materials are under continuous development in an attempt to mimic the extracellular environment of mammalian cells. We have here explored how novel 3D porous scaffolds constructed by polylactic acid, polycaprolactone or chitosan can be decorated with bacterial inclusion bodies, submicron protein particles formed by releasable functional proteins. A simple dipping-based decoration method tested here specifically favors the penetration of the functional particles deeper than 300 μm from the materials' surface. The functionalized surfaces support the intracellular delivery of biologically active proteins to up to more than 80% of the colonizing cells, a process that is slightly influenced by the chemical nature of the scaffold. The combination of 3D soft scaffolds and protein-based sustained release systems (Bioscaffolds) offers promise in the fabrication of bio-inspired hybrid matrices for multifactorial control of cell proliferation in tissue engineering under complex architectonic setting-ups. © 2013 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)63-72
JournalJournal of Controlled Release
Publication statusPublished - 1 Jan 2013


  • 3D scaffolds
  • Bioscaffold
  • Bottom-up delivery
  • Nanoparticles
  • Polylactic acid (PLA)
  • Tissue engineering


Dive into the research topics of 'Functionalization of 3D scaffolds with protein-releasing biomaterials for intracellular delivery'. Together they form a unique fingerprint.

Cite this