TY - JOUR
T1 - Full-genome characterization by deep sequencing of rotavirus A isolates from outbreaks of neonatal diarrhoea in pigs in Spain
AU - Vidal, Anna
AU - Clilverd, Hepzibar
AU - Cortey, Martí
AU - Martín-Valls, Gerard E.
AU - Franzo, Giovanni
AU - Darwich, Laila
AU - Martín, Marga
AU - Mateu, Enric
PY - 2018/12/1
Y1 - 2018/12/1
N2 - © 2018 Elsevier B.V. Since early 2017, in Spain there was an apparent increase in reports on rotavirus involvement in neonatal diarrhoea outbreaks, affecting also adult sows. In this study, 16 unrelated outbreaks of diarrhoea in suckling pigs and sows, where rotavirus A was the only pathogen detected, were investigated. Deep-sequencing was performed on total RNA from twenty-four positive faecal samples. Genotyping, phylogenetic and bayesian analyses showed that all isolates had a common ancestor of porcine, or human porcine-like, origin. The new strain was introduced in the population shortly before the onset of the outbreaks. Besides, a high diversification of the VP7 and VP4 genes occurred in a short time. Isolates presented a high number of amino acid changes in the neutralizing epitopes compared to vaccine sequences. The present report illustrates how a new rotavirus A strain may disseminate rapidly and the extremely high diversification that this pathogen may undergo in a short period.
AB - © 2018 Elsevier B.V. Since early 2017, in Spain there was an apparent increase in reports on rotavirus involvement in neonatal diarrhoea outbreaks, affecting also adult sows. In this study, 16 unrelated outbreaks of diarrhoea in suckling pigs and sows, where rotavirus A was the only pathogen detected, were investigated. Deep-sequencing was performed on total RNA from twenty-four positive faecal samples. Genotyping, phylogenetic and bayesian analyses showed that all isolates had a common ancestor of porcine, or human porcine-like, origin. The new strain was introduced in the population shortly before the onset of the outbreaks. Besides, a high diversification of the VP7 and VP4 genes occurred in a short time. Isolates presented a high number of amino acid changes in the neutralizing epitopes compared to vaccine sequences. The present report illustrates how a new rotavirus A strain may disseminate rapidly and the extremely high diversification that this pathogen may undergo in a short period.
KW - Full genome
KW - NGS
KW - Pig diarrhoea
KW - Rotavirus A
U2 - 10.1016/j.vetmic.2018.10.002
DO - 10.1016/j.vetmic.2018.10.002
M3 - Article
C2 - 30473342
VL - 227
SP - 12
EP - 19
JO - Veterinary Microbiology
JF - Veterinary Microbiology
SN - 0378-1135
ER -
