TY - JOUR
T1 - Freezing of zona‐free mouse embryos: Characteristics of the plasma membrane and subsequent development of the embryos
AU - Martí, M.
AU - Nogués, C.
AU - Egozcue, J.
AU - Ponsà, M.
PY - 1995/1/1
Y1 - 1995/1/1
N2 - Frozen‐thawed mouse embryos with (+ZP) and without (−ZP) zona pellucida have been studied at the Scanning Electron Microscope (SEM) to determine how the process affects the plasma membrane and the subsequent embryo development. The main difference observed in −ZP embryos immediately after thawing is the abnormal morphology and distribution of microvilli. This could explain the spontaneous separation of blastomeres in ‐ZP embryos, and the decrease in their survival rate. If thawed −ZP embryos are allowed to recover in culture, their plasma membrane characteristics and survival rate are identical to those of control embryos. © 1995 Wiley‐Liss, Inc. Copyright © 1995 Wiley‐Liss, Inc., A Wiley Company
AB - Frozen‐thawed mouse embryos with (+ZP) and without (−ZP) zona pellucida have been studied at the Scanning Electron Microscope (SEM) to determine how the process affects the plasma membrane and the subsequent embryo development. The main difference observed in −ZP embryos immediately after thawing is the abnormal morphology and distribution of microvilli. This could explain the spontaneous separation of blastomeres in ‐ZP embryos, and the decrease in their survival rate. If thawed −ZP embryos are allowed to recover in culture, their plasma membrane characteristics and survival rate are identical to those of control embryos. © 1995 Wiley‐Liss, Inc. Copyright © 1995 Wiley‐Liss, Inc., A Wiley Company
UR - https://www.scopus.com/pages/publications/0029644941
U2 - 10.1002/jez.1402720307
DO - 10.1002/jez.1402720307
M3 - Article
SN - 0022-104X
VL - 272
SP - 227
EP - 234
JO - Journal of Experimental Zoology
JF - Journal of Experimental Zoology
IS - 3
ER -