Free-air CO<inf>2</inf> enrichment of wheat: Leaf flavonoid concentration throughout the growth cycle

Marc Estiarte, Josep Peñuelas, Bruce A. Kimball, Donald L. Hendrix, Paul J. Pinter, Gerard W. Wall, Robert L. Lamorte, Douglas J. Hunsaker

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    Abstract

    To test the predictions that plants will have a larger flavonoid concentration in a future world with a CO2-enriched atmosphere, wheat (Triticum aestivum L. cv. Yecora Rojo) was grown in a field experiment using FACE (free-air CO2 enrichment) technology under two levels of atmospheric CO2 concentration: ambient (370 μmol mol-1) and enriched (550 μmol mol-1), and under two levels of irrigation: well-watered (100% replacement of potential evapotranspiration) and half-watered. We also studied the effects of CO2 on the concentration of total non-structural carbohydrates (TNC) and nitrogen (N), two parameters hypothesized to be linked to flavonoid metabolism. Throughout the growth cycle the concentration of isoorientin, the most abundant flavonoid, decreased by 62% (from an average of 12.5 mg g-1 on day of year (DOY) 41 to an average of 4.8 mg g-1 on DOY 123), whereas the concentration of tricin, another characteristic flavone, increased by two orders of magnitude (from an average of 0.007 mg g-1 of isoorientin equivalents on DOY 41 to an average of 0.6 mg g-1 of isoorientin equivalents on DOY 123). Although flavonoid concentration was dependent on growth stage, the effects of treatments on phenology did not invalidate the comparisons between treatments. CO2-enriched plants had higher flavonoid concentrations (14% more isoorientin, an average of 7.0 mg g-1 for ambient CO2 vs an average of 8.0 mg g-1 for enriched CO2), higher TNC concentrations and lower N concentrations in upper canopy leaves throughout the growth cycle. Well-irrigated plants had higher flavonoid concentrations (11% more isoorientin, an average of 7.1 mg g-1 for half watered vs an average of 7.9 mg g-1 for well-watered) throughout the growth cycle, whereas the effect of irrigation treatments on TNC and N was more variable. These results are in accordance with the hypotheses that higher carbon availability promoted by CO2-enrichment provides carbon that can be invested in carbon-based secondary compounds such as flavonoids. The rise in atmospheric CO2 may thus indirectly affect wheat-pest relations, alter the pathogen predisposition and improve the UV-B protection by changing flavonoid concentrations.
    Original languageEnglish
    Pages (from-to)423-433
    JournalPhysiologia Plantarum
    Volume105
    Issue number3
    DOIs
    Publication statusPublished - 1 Mar 1999

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  • Cite this

    Estiarte, M., Peñuelas, J., Kimball, B. A., Hendrix, D. L., Pinter, P. J., Wall, G. W., Lamorte, R. L., & Hunsaker, D. J. (1999). Free-air CO<inf>2</inf> enrichment of wheat: Leaf flavonoid concentration throughout the growth cycle. Physiologia Plantarum, 105(3), 423-433. https://doi.org/10.1034/j.1399-3054.1999.105306.x