In the present report it is shown that histone bands in urea-acetic acid or Triton-urea-acetic acid-polyacrylamide gels can be stained with the fluorescent dye 1-anilinonaphthalene 8-sulfonate and visualized by transillumination of the gel with an uv-light source. The 1-anilinonaphthalene 8-sulfonate staining method described here for urea and Triton-urea gels is rapid (it can be completed in 90 min) and allows the detection of less than 1 μg of histone per band. © 1985.
- 1-anilinonaphthalene 8-sulfonate
- fluorescent staining
- gel staining
- hydrophobic probe
- polyacrylamide gel electrophoresis