Fluorescent properties of histone-1-anilinonaphthalene 8-sulfonate complexes in the presence of denaturant agents: Application to the rapid staining of histones in urea and Triton-urea-polyacrylamide gels

Benjamin Piña, Anna M. Aragay, Pedro Suau, Joan Ramon Daban

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

In the present report it is shown that histone bands in urea-acetic acid or Triton-urea-acetic acid-polyacrylamide gels can be stained with the fluorescent dye 1-anilinonaphthalene 8-sulfonate and visualized by transillumination of the gel with an uv-light source. The 1-anilinonaphthalene 8-sulfonate staining method described here for urea and Triton-urea gels is rapid (it can be completed in 90 min) and allows the detection of less than 1 μg of histone per band. © 1985.
Original languageEnglish
Pages (from-to)431-433
JournalAnalytical Biochemistry
Volume146
Issue number2
DOIs
Publication statusPublished - 1 May 1985

Keywords

  • 1-anilinonaphthalene 8-sulfonate
  • fluorescent staining
  • gel staining
  • histones
  • hydrophobic probe
  • polyacrylamide gel electrophoresis

Fingerprint Dive into the research topics of 'Fluorescent properties of histone-1-anilinonaphthalene 8-sulfonate complexes in the presence of denaturant agents: Application to the rapid staining of histones in urea and Triton-urea-polyacrylamide gels'. Together they form a unique fingerprint.

  • Cite this