In the present report it is shown that histone bands in urea-acetic acid or Triton-urea-acetic acid-polyacrylamide gels can be stained with the fluorescent dye 1-anilinonaphthalene 8-sulfonate and visualized by transillumination of the gel with an uv-light source. The 1-anilinonaphthalene 8-sulfonate staining method described here for urea and Triton-urea gels is rapid (it can be completed in 90 min) and allows the detection of less than 1 μg of histone per band. © 1985.
|Publication status||Published - 1 May 1985|
- 1-anilinonaphthalene 8-sulfonate
- fluorescent staining
- gel staining
- hydrophobic probe
- polyacrylamide gel electrophoresis
Piña, B., Aragay, A. M., Suau, P., & Daban, J. R. (1985). Fluorescent properties of histone-1-anilinonaphthalene 8-sulfonate complexes in the presence of denaturant agents: Application to the rapid staining of histones in urea and Triton-urea-polyacrylamide gels. Analytical Biochemistry, 146(2), 431-433. https://doi.org/10.1016/0003-2697(85)90564-0