Flow injection system based on the sandwich technique for saving expensive reagents

Alberto N. Araujo; JoséL L.F.C. Lima; Julián Alonso-Chamarro; Jordi Bartrolí; Manel Poch

doi:10.1016/0009-8981(91)90157-8

Flow injection system based on the sandwich technique for saving expensive reagents

Alberto N. Araujo, JoséL L.F.C. Lima, Julián Alonso-Chamarro, Jordi Bartrolí, Manel Poch

Department of Chemistry

Research output: Contribution to journal › Article › Research › peer-review

7 Citations (Scopus)

Abstract

We report the application of a sandwich technique in flow injection systems which afford low consumption of expensive reagents and two reagent recirculation systems. The potential applicability of the technique thus developed was assessed by determining glucose in serum samples by the enzymatic glucosidase/peroxidase method. It was possible to perform up to 450 determinations with the same amount of reagent used to perform 50 determinations by batch procedures. The sampling rate was 80 determinations per hour with a 0.9% relative standard deviation. © 1991.

Original language	English
Pages (from-to)	67-76
Journal	Clinica Chimica Acta
Volume	203
Issue number	1
DOIs	https://doi.org/10.1016/0009-8981(91)90157-8
Publication status	Published - 9 Nov 1991

Keywords

8-Port injection valve
Blood serum
Flow injection analysis
Glucose

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abstract = "We report the application of a sandwich technique in flow injection systems which afford low consumption of expensive reagents and two reagent recirculation systems. The potential applicability of the technique thus developed was assessed by determining glucose in serum samples by the enzymatic glucosidase/peroxidase method. It was possible to perform up to 450 determinations with the same amount of reagent used to perform 50 determinations by batch procedures. The sampling rate was 80 determinations per hour with a 0.9% relative standard deviation. {\textcopyright} 1991.",

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AU - Lima, JoséL L.F.C.

AU - Alonso-Chamarro, Julián

AU - Bartrolí, Jordi

AU - Poch, Manel

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AB - We report the application of a sandwich technique in flow injection systems which afford low consumption of expensive reagents and two reagent recirculation systems. The potential applicability of the technique thus developed was assessed by determining glucose in serum samples by the enzymatic glucosidase/peroxidase method. It was possible to perform up to 450 determinations with the same amount of reagent used to perform 50 determinations by batch procedures. The sampling rate was 80 determinations per hour with a 0.9% relative standard deviation. © 1991.

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KW - Flow injection analysis

KW - Glucose

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