Flow cytometry analysis of pituitary adenomas

O. Fornas; M. E. Mato; M. Viader; B. Oliver; P. Tresserres; F. Bartumeus; D. Tugues; J. Pétriz; X. Matías-Guiu; S. M. Webb

doi:10.1159/000185097

Flow cytometry analysis of pituitary adenomas

O. Fornas, M. E. Mato, M. Viader, B. Oliver, P. Tresserres, F. Bartumeus, D. Tugues, J. Pétriz, X. Matías-Guiu, S. M. Webb

Research output: Contribution to journal › Article › Research › peer-review

6 Citations (Scopus)

Abstract

Using flow cytometry, DNA content and index, and/or proliferative capacity (measuring proliferating cell nuclear antigen PCNA) in operated pituitary tumors, control pituitaries obtained at necropsy, and experimental pituitary hyperplasia induced in rats were analyzed. Simultaneous measurement of cell ploidy and proliferation differentiated normal pituitary (diploid DNA index and negative PCNA) from pituitary hyperplasia (diploid DNA index with intensely positive PCNA, between 30 and 72% of cells). In the tumors 83% (19/ 23) were positive for PCNA (between 3 and 84%) and 73% (17/23) aneuploid; only 1 tumor was diploid and negative for PCNA. Conclusions: Differentiation between normal and abnormal (neoplastic or hyperplastic) pituitary is possible by flow cytometry, but in the adenomas no correlation with postoperative clinical outcome was observed. © 1996 S. Karger AG, Basel.

Original language	English
Pages (from-to)	257-262
Journal	Hormone Research in Paediatrics
Volume	46
Issue number	6
DOIs	https://doi.org/10.1159/000185097
Publication status	Published - 1 Jan 1996

Keywords

Cell proliferation
Flow cytometry
Pituitary adenomas
Ploidy
Prognosis
Treatment

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10.1159/000185097

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title = "Flow cytometry analysis of pituitary adenomas",

abstract = "Using flow cytometry, DNA content and index, and/or proliferative capacity (measuring proliferating cell nuclear antigen PCNA) in operated pituitary tumors, control pituitaries obtained at necropsy, and experimental pituitary hyperplasia induced in rats were analyzed. Simultaneous measurement of cell ploidy and proliferation differentiated normal pituitary (diploid DNA index and negative PCNA) from pituitary hyperplasia (diploid DNA index with intensely positive PCNA, between 30 and 72% of cells). In the tumors 83% (19/ 23) were positive for PCNA (between 3 and 84%) and 73% (17/23) aneuploid; only 1 tumor was diploid and negative for PCNA. Conclusions: Differentiation between normal and abnormal (neoplastic or hyperplastic) pituitary is possible by flow cytometry, but in the adenomas no correlation with postoperative clinical outcome was observed. {\textcopyright} 1996 S. Karger AG, Basel.",

keywords = "Cell proliferation, Flow cytometry, Pituitary adenomas, Ploidy, Prognosis, Treatment",

author = "O. Fornas and Mato, {M. E.} and M. Viader and B. Oliver and P. Tresserres and F. Bartumeus and D. Tugues and J. P{\'e}triz and X. Mat{\'i}as-Guiu and Webb, {S. M.}",

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doi = "10.1159/000185097",

language = "English",

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TY - JOUR

T1 - Flow cytometry analysis of pituitary adenomas

AU - Fornas, O.

AU - Mato, M. E.

AU - Viader, M.

AU - Oliver, B.

AU - Tresserres, P.

AU - Bartumeus, F.

AU - Tugues, D.

AU - Pétriz, J.

AU - Matías-Guiu, X.

AU - Webb, S. M.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Using flow cytometry, DNA content and index, and/or proliferative capacity (measuring proliferating cell nuclear antigen PCNA) in operated pituitary tumors, control pituitaries obtained at necropsy, and experimental pituitary hyperplasia induced in rats were analyzed. Simultaneous measurement of cell ploidy and proliferation differentiated normal pituitary (diploid DNA index and negative PCNA) from pituitary hyperplasia (diploid DNA index with intensely positive PCNA, between 30 and 72% of cells). In the tumors 83% (19/ 23) were positive for PCNA (between 3 and 84%) and 73% (17/23) aneuploid; only 1 tumor was diploid and negative for PCNA. Conclusions: Differentiation between normal and abnormal (neoplastic or hyperplastic) pituitary is possible by flow cytometry, but in the adenomas no correlation with postoperative clinical outcome was observed. © 1996 S. Karger AG, Basel.

AB - Using flow cytometry, DNA content and index, and/or proliferative capacity (measuring proliferating cell nuclear antigen PCNA) in operated pituitary tumors, control pituitaries obtained at necropsy, and experimental pituitary hyperplasia induced in rats were analyzed. Simultaneous measurement of cell ploidy and proliferation differentiated normal pituitary (diploid DNA index and negative PCNA) from pituitary hyperplasia (diploid DNA index with intensely positive PCNA, between 30 and 72% of cells). In the tumors 83% (19/ 23) were positive for PCNA (between 3 and 84%) and 73% (17/23) aneuploid; only 1 tumor was diploid and negative for PCNA. Conclusions: Differentiation between normal and abnormal (neoplastic or hyperplastic) pituitary is possible by flow cytometry, but in the adenomas no correlation with postoperative clinical outcome was observed. © 1996 S. Karger AG, Basel.

KW - Cell proliferation

KW - Flow cytometry

KW - Pituitary adenomas

KW - Ploidy

KW - Prognosis

KW - Treatment

U2 - 10.1159/000185097

DO - 10.1159/000185097

M3 - Article

VL - 46

SP - 257

EP - 262

IS - 6

ER -

Flow cytometry analysis of pituitary adenomas

Abstract

Keywords

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