TY - JOUR
T1 - First Description of blaNDM-7 Carried on an IncX4 Plasmid in Escherichia coli ST679 Isolated in Spain.
AU - Llovet Pellejero, Maria Teresa
AU - Espinal, Paula
AU - Miro Cardona, Elisenda
AU - Segura Alvarez, M. Concepcion
AU - Gomez, Laura
AU - Plasencia Miguel, Maria Virginia
AU - Coll Figa, Pere, null
AU - Navarro Risueño, Ferran
PY - 2018/3/1
Y1 - 2018/3/1
N2 - © Copyright 2018, Mary Ann Liebert, Inc. 2018. This study describes the molecular characterization of an NDM-7 carbapenemase-producing Escherichia coli strain Ec188, recovered from a rectal swab of a male patient who had travelled to Pakistan before his hospitalization at the Hospital del Mar in Barcelona, Spain. The Ec188 isolate, assigned to a new multilocus sequence type ST679, was resistant to all beta-lactams, aminoglycosides (gentamicin, tobramycin, and with reduced susceptibility to amikacin), and ciprofloxacin. The blaNDM-7 gene was located on a 50 kb IncX4 plasmid (pEc188-NDM7), both in the original and transconjugant strains. In addition, blaCTX-M-15 was located on a 150 kb IncFIA plasmid and blaCMY-2 on a 95 kb undetermined plasmid type, only in the wild-type strain. The immediate genetic surroundings of blaNDM-7 included the bleo, trpf, and dsbC genes, and it was flanked by the insertion sequences IS26 and ISAba125, which appeared interrupted by IS5. The res and parA genes were found in the same orientation downstream of the IS26 element. To our knowledge, this is the first report of an NDM-7-carbapenemase carried on an IncX4 plasmid, as well as the first E. coli strain belonging to ST679 harboring an NDM β-lactamase, possibly associated with previous travel to Pakistan. In addition, this study highlights the dissemination of NDM variants accompanied by IncX-type plasmids.
AB - © Copyright 2018, Mary Ann Liebert, Inc. 2018. This study describes the molecular characterization of an NDM-7 carbapenemase-producing Escherichia coli strain Ec188, recovered from a rectal swab of a male patient who had travelled to Pakistan before his hospitalization at the Hospital del Mar in Barcelona, Spain. The Ec188 isolate, assigned to a new multilocus sequence type ST679, was resistant to all beta-lactams, aminoglycosides (gentamicin, tobramycin, and with reduced susceptibility to amikacin), and ciprofloxacin. The blaNDM-7 gene was located on a 50 kb IncX4 plasmid (pEc188-NDM7), both in the original and transconjugant strains. In addition, blaCTX-M-15 was located on a 150 kb IncFIA plasmid and blaCMY-2 on a 95 kb undetermined plasmid type, only in the wild-type strain. The immediate genetic surroundings of blaNDM-7 included the bleo, trpf, and dsbC genes, and it was flanked by the insertion sequences IS26 and ISAba125, which appeared interrupted by IS5. The res and parA genes were found in the same orientation downstream of the IS26 element. To our knowledge, this is the first report of an NDM-7-carbapenemase carried on an IncX4 plasmid, as well as the first E. coli strain belonging to ST679 harboring an NDM β-lactamase, possibly associated with previous travel to Pakistan. In addition, this study highlights the dissemination of NDM variants accompanied by IncX-type plasmids.
KW - Enterobacteriaceae
KW - antimicrobial resistance
KW - carbapenemases
KW - genetic mobile elements
UR - http://europepmc.org/abstract/med/28650275
U2 - 10.1089/mdr.2017.0039
DO - 10.1089/mdr.2017.0039
M3 - Article
C2 - 28650275
SN - 1076-6294
VL - 24
SP - 113
EP - 119
JO - Microbial Drug Resistance
JF - Microbial Drug Resistance
IS - 2
ER -