Abstract
An FIA method for the determination of calcium pantothenate, one of the components of vitamin B complex, is proposed. The method is based on the alkaline hydrolysis of the analyte to B-alanine and subsequent derivatization to a l-alkylthio-2-alkylisoindole, the fluorescence of which is monitored at x — 350 nm and Xern = 455 nm. Chemical and physical variables influencing the fluorescence signal were optimized and the method was validated as regards accuracy, repeatability and reproducibility. The proposed method features a broad linear determination range (0.2-40 mg/L) and a throughput of 25 samples/h. It was applied to the determination of calcium pantothenate in multivitamin preparations with satisfactory results. © 1995, Taylor & Francis Group, LLC. All rights reserved.
Original language | English |
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Pages (from-to) | 821-833 |
Journal | Analytical Letters |
Volume | 28 |
Issue number | 5 |
DOIs | |
Publication status | Published - 1 Jan 1995 |
Keywords
- FIA
- Fluorimetric detection
- Multivitamin preparations
- Pantothenate