Fia Fluorimetric Determination Of Calcium Pantothenate. Validation And Quantitation In Multivitamin Preparations

M. Blanco, J. Coello, H. Iturriaga, S. Maspoch, J. Pages

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3 Citations (Scopus)

Abstract

An FIA method for the determination of calcium pantothenate, one of the components of vitamin B complex, is proposed. The method is based on the alkaline hydrolysis of the analyte to B-alanine and subsequent derivatization to a l-alkylthio-2-alkylisoindole, the fluorescence of which is monitored at x — 350 nm and Xern = 455 nm. Chemical and physical variables influencing the fluorescence signal were optimized and the method was validated as regards accuracy, repeatability and reproducibility. The proposed method features a broad linear determination range (0.2-40 mg/L) and a throughput of 25 samples/h. It was applied to the determination of calcium pantothenate in multivitamin preparations with satisfactory results. © 1995, Taylor & Francis Group, LLC. All rights reserved.
Original languageEnglish
Pages (from-to)821-833
JournalAnalytical Letters
Volume28
Issue number5
DOIs
Publication statusPublished - 1 Jan 1995

Keywords

  • FIA
  • Fluorimetric detection
  • Multivitamin preparations
  • Pantothenate

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