Fia Fluorimetric Determination Of Calcium Pantothenate. Validation And Quantitation In Multivitamin Preparations

M. Blanco; J. Coello; H. Iturriaga; S. Maspoch; J. Pages

doi:10.1080/00032719508001427

Fia Fluorimetric Determination Of Calcium Pantothenate. Validation And Quantitation In Multivitamin Preparations

M. Blanco, J. Coello, H. Iturriaga, S. Maspoch, J. Pages

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Abstract

An FIA method for the determination of calcium pantothenate, one of the components of vitamin B complex, is proposed. The method is based on the alkaline hydrolysis of the analyte to B-alanine and subsequent derivatization to a l-alkylthio-2-alkylisoindole, the fluorescence of which is monitored at x — 350 nm and Xern = 455 nm. Chemical and physical variables influencing the fluorescence signal were optimized and the method was validated as regards accuracy, repeatability and reproducibility. The proposed method features a broad linear determination range (0.2-40 mg/L) and a throughput of 25 samples/h. It was applied to the determination of calcium pantothenate in multivitamin preparations with satisfactory results. © 1995, Taylor & Francis Group, LLC. All rights reserved.

Original language	English
Pages (from-to)	821-833
Journal	Analytical Letters
Volume	28
Issue number	5
DOIs	https://doi.org/10.1080/00032719508001427
Publication status	Published - 1 Jan 1995

Keywords

FIA
Fluorimetric detection
Multivitamin preparations
Pantothenate

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10.1080/00032719508001427

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title = "Fia Fluorimetric Determination Of Calcium Pantothenate. Validation And Quantitation In Multivitamin Preparations",

abstract = "An FIA method for the determination of calcium pantothenate, one of the components of vitamin B complex, is proposed. The method is based on the alkaline hydrolysis of the analyte to B-alanine and subsequent derivatization to a l-alkylthio-2-alkylisoindole, the fluorescence of which is monitored at x — 350 nm and Xern = 455 nm. Chemical and physical variables influencing the fluorescence signal were optimized and the method was validated as regards accuracy, repeatability and reproducibility. The proposed method features a broad linear determination range (0.2-40 mg/L) and a throughput of 25 samples/h. It was applied to the determination of calcium pantothenate in multivitamin preparations with satisfactory results. {\textcopyright} 1995, Taylor & Francis Group, LLC. All rights reserved.",

keywords = "FIA, Fluorimetric detection, Multivitamin preparations, Pantothenate",

author = "M. Blanco and J. Coello and H. Iturriaga and S. Maspoch and J. Pages",

year = "1995",

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doi = "10.1080/00032719508001427",

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TY - JOUR

T1 - Fia Fluorimetric Determination Of Calcium Pantothenate. Validation And Quantitation In Multivitamin Preparations

AU - Blanco, M.

AU - Coello, J.

AU - Iturriaga, H.

AU - Maspoch, S.

AU - Pages, J.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - An FIA method for the determination of calcium pantothenate, one of the components of vitamin B complex, is proposed. The method is based on the alkaline hydrolysis of the analyte to B-alanine and subsequent derivatization to a l-alkylthio-2-alkylisoindole, the fluorescence of which is monitored at x — 350 nm and Xern = 455 nm. Chemical and physical variables influencing the fluorescence signal were optimized and the method was validated as regards accuracy, repeatability and reproducibility. The proposed method features a broad linear determination range (0.2-40 mg/L) and a throughput of 25 samples/h. It was applied to the determination of calcium pantothenate in multivitamin preparations with satisfactory results. © 1995, Taylor & Francis Group, LLC. All rights reserved.

AB - An FIA method for the determination of calcium pantothenate, one of the components of vitamin B complex, is proposed. The method is based on the alkaline hydrolysis of the analyte to B-alanine and subsequent derivatization to a l-alkylthio-2-alkylisoindole, the fluorescence of which is monitored at x — 350 nm and Xern = 455 nm. Chemical and physical variables influencing the fluorescence signal were optimized and the method was validated as regards accuracy, repeatability and reproducibility. The proposed method features a broad linear determination range (0.2-40 mg/L) and a throughput of 25 samples/h. It was applied to the determination of calcium pantothenate in multivitamin preparations with satisfactory results. © 1995, Taylor & Francis Group, LLC. All rights reserved.

KW - FIA

KW - Fluorimetric detection

KW - Multivitamin preparations

KW - Pantothenate

U2 - 10.1080/00032719508001427

DO - 10.1080/00032719508001427

M3 - Article

VL - 28

SP - 821

EP - 833

IS - 5

ER -

Fia Fluorimetric Determination Of Calcium Pantothenate. Validation And Quantitation In Multivitamin Preparations

Abstract

Keywords

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