An FIA method for the determination of calcium pantothenate, one of the components of vitamin B complex, is proposed. The method is based on the alkaline hydrolysis of the analyte to B-alanine and subsequent derivatization to a l-alkylthio-2-alkylisoindole, the fluorescence of which is monitored at x — 350 nm and Xern = 455 nm. Chemical and physical variables influencing the fluorescence signal were optimized and the method was validated as regards accuracy, repeatability and reproducibility. The proposed method features a broad linear determination range (0.2-40 mg/L) and a throughput of 25 samples/h. It was applied to the determination of calcium pantothenate in multivitamin preparations with satisfactory results. © 1995, Taylor & Francis Group, LLC. All rights reserved.
|Publication status||Published - 1 Jan 1995|
- Fluorimetric detection
- Multivitamin preparations