FGF-4 increases in vitro expansion rate of human adult bone marrow-derived mesenchymal stem cells

Jordi Farré; Santiago Roura; Cristina Prat-Vidal; Carolina Soler-Botija; Anna Llach; Cristina E. Molina; Leif Hove-Madsen; Jordi J. Cairó; Francesc Gòdia; Ramon Bragós; Juan Cinca; Antoni Bayes-Genis

doi:https://doi.org/10.1080/08977190701345200

FGF-4 increases in vitro expansion rate of human adult bone marrow-derived mesenchymal stem cells

Jordi Farré, Santiago Roura, Cristina Prat-Vidal, Carolina Soler-Botija, Anna Llach, Cristina E. Molina, Leif Hove-Madsen, Jordi J. Cairó, Francesc Gòdia, Ramon Bragós, Juan Cinca, Antoni Bayes-Genis

Department of Medicine

Research output: Contribution to journal › Article › Research › peer-review

44 Citations (Scopus)

Abstract

Human bone marrow-derived mesenchymal stem cells (MSCs) exhibit limited in vitro growth. Fibroblast growth factors (FGFs) elicit a variety of biological responses, such as cell proliferation, differentiation and migration. FGF-4 represents one of the FGFs with the highest cell mitogenic activity. We studied the effect of FGF-4 on MSCs growth and pluripotency. MSCs duplication time (Td) was significantly reduced with FGF-4 compared to controls (2.2 ± 0.2 vs. 4.1 ± 0.2 days, respectively; p = 0.03) while BMP-2 and SCF-1 did not exert a significant growth effect. MSC expression of surface markers, differentiation into adipogenic and osteogenic lineages, and baseline expression of cardiomyogenic genes were unaffected by FGF-4. In summary, exogenous FGF-4 increases the rate at which MSC proliferate and has no significant effect on MSC pluripotency.

Original language	English
Pages (from-to)	71-76
Journal	Growth Factors
Volume	25
DOIs	https://doi.org/10.1080/08977190701345200
Publication status	Published - 1 Apr 2007

Keywords

Cardiomyogenic
FGF-4
Growth
Mesenchymal stem cells
Pluripotency

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title = "FGF-4 increases in vitro expansion rate of human adult bone marrow-derived mesenchymal stem cells",

abstract = "Human bone marrow-derived mesenchymal stem cells (MSCs) exhibit limited in vitro growth. Fibroblast growth factors (FGFs) elicit a variety of biological responses, such as cell proliferation, differentiation and migration. FGF-4 represents one of the FGFs with the highest cell mitogenic activity. We studied the effect of FGF-4 on MSCs growth and pluripotency. MSCs duplication time (Td) was significantly reduced with FGF-4 compared to controls (2.2 ± 0.2 vs. 4.1 ± 0.2 days, respectively; p = 0.03) while BMP-2 and SCF-1 did not exert a significant growth effect. MSC expression of surface markers, differentiation into adipogenic and osteogenic lineages, and baseline expression of cardiomyogenic genes were unaffected by FGF-4. In summary, exogenous FGF-4 increases the rate at which MSC proliferate and has no significant effect on MSC pluripotency.",

keywords = "Cardiomyogenic, FGF-4, Growth, Mesenchymal stem cells, Pluripotency",

author = "Jordi Farr{\'e} and Santiago Roura and Cristina Prat-Vidal and Carolina Soler-Botija and Anna Llach and Molina, {Cristina E.} and Leif Hove-Madsen and Cair{\'o}, {Jordi J.} and Francesc G{\`o}dia and Ramon Brag{\'o}s and Juan Cinca and Antoni Bayes-Genis",

year = "2007",

month = apr,

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doi = "https://doi.org/10.1080/08977190701345200",

language = "English",

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TY - JOUR

T1 - FGF-4 increases in vitro expansion rate of human adult bone marrow-derived mesenchymal stem cells

AU - Farré, Jordi

AU - Roura, Santiago

AU - Prat-Vidal, Cristina

AU - Soler-Botija, Carolina

AU - Llach, Anna

AU - Molina, Cristina E.

AU - Hove-Madsen, Leif

AU - Cairó, Jordi J.

AU - Gòdia, Francesc

AU - Bragós, Ramon

AU - Cinca, Juan

AU - Bayes-Genis, Antoni

PY - 2007/4/1

Y1 - 2007/4/1

N2 - Human bone marrow-derived mesenchymal stem cells (MSCs) exhibit limited in vitro growth. Fibroblast growth factors (FGFs) elicit a variety of biological responses, such as cell proliferation, differentiation and migration. FGF-4 represents one of the FGFs with the highest cell mitogenic activity. We studied the effect of FGF-4 on MSCs growth and pluripotency. MSCs duplication time (Td) was significantly reduced with FGF-4 compared to controls (2.2 ± 0.2 vs. 4.1 ± 0.2 days, respectively; p = 0.03) while BMP-2 and SCF-1 did not exert a significant growth effect. MSC expression of surface markers, differentiation into adipogenic and osteogenic lineages, and baseline expression of cardiomyogenic genes were unaffected by FGF-4. In summary, exogenous FGF-4 increases the rate at which MSC proliferate and has no significant effect on MSC pluripotency.

AB - Human bone marrow-derived mesenchymal stem cells (MSCs) exhibit limited in vitro growth. Fibroblast growth factors (FGFs) elicit a variety of biological responses, such as cell proliferation, differentiation and migration. FGF-4 represents one of the FGFs with the highest cell mitogenic activity. We studied the effect of FGF-4 on MSCs growth and pluripotency. MSCs duplication time (Td) was significantly reduced with FGF-4 compared to controls (2.2 ± 0.2 vs. 4.1 ± 0.2 days, respectively; p = 0.03) while BMP-2 and SCF-1 did not exert a significant growth effect. MSC expression of surface markers, differentiation into adipogenic and osteogenic lineages, and baseline expression of cardiomyogenic genes were unaffected by FGF-4. In summary, exogenous FGF-4 increases the rate at which MSC proliferate and has no significant effect on MSC pluripotency.

KW - Cardiomyogenic

KW - FGF-4

KW - Growth

KW - Mesenchymal stem cells

KW - Pluripotency

U2 - https://doi.org/10.1080/08977190701345200

DO - https://doi.org/10.1080/08977190701345200

M3 - Article

VL - 25

SP - 71

EP - 76

ER -

FGF-4 increases in vitro expansion rate of human adult bone marrow-derived mesenchymal stem cells

Abstract

Keywords

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