In order to suggest a production process that reduces fermentation time and supplies the proper characteristics to the culture broth for a better recovery of lipase, the influence of three parameters over lipase production by Candida rugosa has been studied. It has been found that oxygen deficiency restricts lipase production by this microorganism, while a shortage of nitrogen source seems to have no effect on lipase production when glucose and olive oil are used jointly as carbon sources. Among the substrates tested, the best results are obtained with the use of olive oil as carbon source. Not only does this procedure provide the highest lipase production found in this work, but this production is also related with the growth. © 1991.
|Journal||Journal of Fermentation and Bioengineering|
|Publication status||Published - 1 Jan 1991|