TY - JOUR
T1 - Fed-batch operational strategies for recombinant Fab production with Pichia pastoris using the constitutive GAP promoter
AU - Garcia-Ortega, Xavier
AU - Ferrer, Pau
AU - Montesinos, José Luis
AU - Valero, Francisco
PY - 2013/10/5
Y1 - 2013/10/5
N2 - Carbon source and growth rate are two major parameters affecting recombinant protein production in Pichia pastoris. The effect of the most commonly used carbon sources (glycerol or glucose) and the specific growth rate (. μ) has been studied on the production of a human antigen-binding fragment (Fab) in this cell factory under the constitutive GAP promoter in fed-batch cultures.Glycerol for batch phase and glucose for fed-batch phase was the most successful carbon source combination. During batch phase, by-products were detected when glucose was used, despite maintaining DO at values higher than 35%. Also, the presence of cell aggregates was detected affecting the reproducibility and operability of the bioprocess.Conversely, glucose was the best substrate for fed-batch phase. When working at C-limiting conditions, neither by-products nor aggregates were detected and Fab production levels were comparable to those obtained with glycerol. In addition, the lower heat yield (YQ/X) and oxygen to biomass yield (YO2/X) for glucose-supported cultures made this substrate the best alternative from an industrial operational point of view.In addition, the effect of specific growth rate on fed-batch Fab production was studied. Medium and high μ (0.10 and 0.15h-1) set-points showed similar Fab production yield. However, in terms of total and volumetric productivity, higher μ was the best process condition. © 2013 Elsevier B.V.
AB - Carbon source and growth rate are two major parameters affecting recombinant protein production in Pichia pastoris. The effect of the most commonly used carbon sources (glycerol or glucose) and the specific growth rate (. μ) has been studied on the production of a human antigen-binding fragment (Fab) in this cell factory under the constitutive GAP promoter in fed-batch cultures.Glycerol for batch phase and glucose for fed-batch phase was the most successful carbon source combination. During batch phase, by-products were detected when glucose was used, despite maintaining DO at values higher than 35%. Also, the presence of cell aggregates was detected affecting the reproducibility and operability of the bioprocess.Conversely, glucose was the best substrate for fed-batch phase. When working at C-limiting conditions, neither by-products nor aggregates were detected and Fab production levels were comparable to those obtained with glycerol. In addition, the lower heat yield (YQ/X) and oxygen to biomass yield (YO2/X) for glucose-supported cultures made this substrate the best alternative from an industrial operational point of view.In addition, the effect of specific growth rate on fed-batch Fab production was studied. Medium and high μ (0.10 and 0.15h-1) set-points showed similar Fab production yield. However, in terms of total and volumetric productivity, higher μ was the best process condition. © 2013 Elsevier B.V.
KW - Antibody fragment
KW - Fed-batch
KW - P GAP
KW - Pichia pastoris
KW - Recombinant protein production
KW - Specific growth rate
KW - Substrates
U2 - https://doi.org/10.1016/j.bej.2013.07.013
DO - https://doi.org/10.1016/j.bej.2013.07.013
M3 - Article
VL - 79
SP - 172
EP - 181
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
SN - 1369-703X
ER -