Extracellular-Vesicle Isolation from Different Biological Fluids by Size-Exclusion Chromatography

Marta Monguió-Tortajada, Miriam Morón-Font, Ana Gámez-Valero, Laura Carreras-Planella, Francesc E. Borràs, Marcella Franquesa

Research output: Contribution to journalArticleResearch

20 Citations (Scopus)

Abstract

© 2019 John Wiley & Sons, Inc. This unit describes how to isolate extracellular vesicles (EVs) from different biological fluids using size-exclusion chromatography (SEC) and how to prepare your starting sample and the EV product for downstream applications. EVs are membrane nanovesicles with specific content that reflects the phenotype and functions of the cell of origin, including protected proteins, lipids, metabolites, and nucleic acids. EVs are thus an excellent resource for noninvasive biomarker discovery in a number of pathological situations and are a promising nanotherapeutic tool to overcome the disadvantages associated with cellular therapy. However, there are still no standardized methods to isolate pure EV preparations, as many approaches do not guarantee proper EV purification, free of contaminating non-EV molecules. Currently, SEC is one of the most promising approaches to purify EVs from any biological fluid, as it avoids co-isolation of contaminants and is user friendly and scalable. © 2019 by John Wiley & Sons, Inc.
Original languageEnglish
Article numbere82
JournalCurrent Protocols in Stem Cell Biology
Volume49
DOIs
Publication statusPublished - 1 Jun 2019

Keywords

  • conditioned medium
  • dialysis
  • exosome
  • plasma
  • urine

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