Extender osmolality, glycerol and egg yolk on the cryopreservation of epididymal spermatozoa for gamete banking of the Cantabric Chamois (Rupicapra pyrenaica parva)

Felipe Martínez-Pastor, Mercedes Álvarez, Camino Guerra, César A. Chamorro, Luis Anel-López, Paulino de Paz, Luis Anel, Manuel Álvarez-Rodríguez

    Research output: Contribution to journalArticleResearch

    Abstract

    © 2018 Elsevier Inc. Germplasm banking is a key technology enabling the ex-situ conservation of wild species. However, cryopreservation protocols must be tested to assure the applicability of the banked material. The objective of this study was defining a range of parameters for the composition of a semen extender for Cantabrian chamois epididymal spermatozoa (post-mortem collection). The freezing extender was based in a TES-Tris-fructose buffer, modifying its composition in three experiments: Osmolality of the buffer (320, 380 or 430 mOsm/kg, 8% glycerol, 15% egg yolk), glycerol (4 or 8%, 430 mOsm/kg, 15% egg yolk), egg yolk (10 or 15%, 430 mOsm/kg, 4% glycerol). Sperm was extended at 100 mill. spermatozoa/ml, cooled at 5 °C and frozen at −20 °C/min. Sperm quality was assessed pre and post-thawing (CASA, HOS test, abnormal forms, cytoplasmic droplets, and viability and acrosomal damage by flow cytometry). Freezability was good overall, with total motility of 65.5% ± 2.4 initial and 55.8% ± 2.4 post-thawing. The extenders affected the post-thaw sperm quality marginally. Whereas osmolalities and glycerol concentrations seemed not to differ, 430 mOsm/kg and 4% glycerol might be preferred. Egg yolk concentrations only differed on sperm velocity (VCL: 84.0 ± 6.7 μm/s in 10% vs. 70.7 ± 6.2 μm/s in 15%, P < 0.05). Our results suggest a good cryotolerance of chamois epididymal spermatozoa, with a preferred extender composition of hyperosmotic buffer, glycerol in the 4% range and lower egg yolk (10% range) than other ruminants.
    Original languageEnglish
    Pages (from-to)109-114
    JournalTheriogenology
    Volume125
    DOIs
    Publication statusPublished - 1 Feb 2019

    Keywords

    • Cantabrian chamois
    • Cryopreservation
    • Epididymal spermatozoa
    • Extender
    • Glycerol
    • Osmolality

    Fingerprint Dive into the research topics of 'Extender osmolality, glycerol and egg yolk on the cryopreservation of epididymal spermatozoa for gamete banking of the Cantabric Chamois (Rupicapra pyrenaica parva)'. Together they form a unique fingerprint.

  • Cite this

    Martínez-Pastor, F., Álvarez, M., Guerra, C., Chamorro, C. A., Anel-López, L., de Paz, P., Anel, L., & Álvarez-Rodríguez, M. (2019). Extender osmolality, glycerol and egg yolk on the cryopreservation of epididymal spermatozoa for gamete banking of the Cantabric Chamois (Rupicapra pyrenaica parva). Theriogenology, 125, 109-114. https://doi.org/10.1016/j.theriogenology.2018.10.022