Extended-spectrum β-lactamase-producing Enterobacteriaceae in different environments (humans, food, animal farms and sewage)

Raúl Jesús Mesa, Vanessa Blanc, Anicet R. Blanch, Pilar Cortés, Juan José González, Susana Lavilla, Elisenda Miró, Maite Muniesa, Montserrat Saco, Ma Teresa Tórtola, Beatriz Mirelis, Pere Coll, Montserrat Llagostera, Guillem Prats, Ferran Navarro

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171 Citations (Scopus)

Abstract

Objectives: This study aimed to determine the presence of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae in different environments. Methods: Clinical samples and stool samples from animal farms, sewage, human faecal carriers attending the emergency room and faecal carriers in the context of food-borne disease outbreaks were subcultured onto MacConkey agar supplemented with cefotaxime for the detection of ESBL-producing Enterobacteriaceae. Identification, susceptibility pattern and ERIC-PCR were used for clone delineation in each sample. Community consumption of antibiotics was also recorded. Results: An ESBL-producing Enterobacteriaceae prevalence of 1.9% was observed in human infections. A cross-sectional survey of human faecal carriers in the community showed a general prevalence of 6.6% with a temporal distribution. High use of antibiotics in winter coincided with a lower prevalence in carriers. ESBL-producing Enterobacteriaceae were detected in the five samples of human sewage, in samples from 8 of 10 pig farms, 2 of 10 rabbit farms, from all 10 poultry farms and in 3 of 738 food samples studied. Faecal carriage of ESBL-producing Enterobacteriaceae was detected in samples from 19 of 61 food-borne outbreaks evaluated. All food-borne outbreaks were due to enteropathogens. The prevalence of carriers in these outbreaks ranged from 4.4% to 66.6%. Conclusions: This widespread occurrence of ESBL-producing Enterobacteriaceae suggests that the community could act as a reservoir and that food could contribute to the spread of these strains. © 2006 Oxford University Press.
Original languageEnglish
Pages (from-to)211-215
JournalJournal of Antimicrobial Chemotherapy
Volume58
DOIs
Publication statusPublished - 1 Jul 2006

Keywords

  • Drug resistance
  • ESBLs
  • Farms
  • Food outbreaks
  • Sewage
  • β-lactamases

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