Expression of the Pseudomonas aeruginosa uvrA gene is constitutive

Eusebi Rivera; Laura Vila; Jordi Barbé

doi:https://doi.org/10.1016/S0027-5107(97)00061-4

Expression of the Pseudomonas aeruginosa uvrA gene is constitutive

Eusebi Rivera, Laura Vila, Jordi Barbé

Department of Genetics and Microbiology

Research output: Contribution to journal › Article › Research › peer-review

16 Citations (Scopus)

Abstract

By complementation of an Escherichia coli uvrA-defective mutant, the uvrA gene of Pseudomonas aeruginosa has been isolated from a genomic library. The nucleotide sequence of the promoter and the 5'-coding region of this gene have been determined. A P. aeruginosa SOS consensus region, which functions as a binding site for the LexA repressor molecule, was not present in the 629-bp upstream region of the cloned uvrA gene. Analysis of transcriptional fusions with the lacZ gene demonstrates that, contrary to what happens with its homologous gene of E. coli, the P. aeruginosa uvrA gene is not DNA damage-inducible. However, the uvrA protein must be functional in P. aeruginosa cells because an uvrA-defective mutant is extremely sensitive to UV radiation.

Original language	English
Pages (from-to)	149-155
Journal	Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume	377
DOIs	https://doi.org/10.1016/S0027-5107(97)00061-4
Publication status	Published - 9 Jun 1997

Keywords

DNA damage
Pseudomonas aeruginosa
SOS system
uvrA gene

Access to Document

https://doi.org/10.1016/S0027-5107(97)00061-4

Fingerprint Dive into the research topics of 'Expression of the Pseudomonas aeruginosa uvrA gene is constitutive'. Together they form a unique fingerprint.

Cite this

@article{7c94497e0bcd4e8bb71beb7b5ddf1fed,

title = "Expression of the Pseudomonas aeruginosa uvrA gene is constitutive",

abstract = "By complementation of an Escherichia coli uvrA-defective mutant, the uvrA gene of Pseudomonas aeruginosa has been isolated from a genomic library. The nucleotide sequence of the promoter and the 5'-coding region of this gene have been determined. A P. aeruginosa SOS consensus region, which functions as a binding site for the LexA repressor molecule, was not present in the 629-bp upstream region of the cloned uvrA gene. Analysis of transcriptional fusions with the lacZ gene demonstrates that, contrary to what happens with its homologous gene of E. coli, the P. aeruginosa uvrA gene is not DNA damage-inducible. However, the uvrA protein must be functional in P. aeruginosa cells because an uvrA-defective mutant is extremely sensitive to UV radiation.",

keywords = "DNA damage, Pseudomonas aeruginosa, SOS system, uvrA gene",

author = "Eusebi Rivera and Laura Vila and Jordi Barb{\'e}",

year = "1997",

month = jun,

day = "9",

doi = "https://doi.org/10.1016/S0027-5107(97)00061-4",

language = "English",

volume = "377",

pages = "149--155",

journal = "Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis",

issn = "0027-5107",

}

TY - JOUR

T1 - Expression of the Pseudomonas aeruginosa uvrA gene is constitutive

AU - Rivera, Eusebi

AU - Vila, Laura

AU - Barbé, Jordi

PY - 1997/6/9

Y1 - 1997/6/9

N2 - By complementation of an Escherichia coli uvrA-defective mutant, the uvrA gene of Pseudomonas aeruginosa has been isolated from a genomic library. The nucleotide sequence of the promoter and the 5'-coding region of this gene have been determined. A P. aeruginosa SOS consensus region, which functions as a binding site for the LexA repressor molecule, was not present in the 629-bp upstream region of the cloned uvrA gene. Analysis of transcriptional fusions with the lacZ gene demonstrates that, contrary to what happens with its homologous gene of E. coli, the P. aeruginosa uvrA gene is not DNA damage-inducible. However, the uvrA protein must be functional in P. aeruginosa cells because an uvrA-defective mutant is extremely sensitive to UV radiation.

AB - By complementation of an Escherichia coli uvrA-defective mutant, the uvrA gene of Pseudomonas aeruginosa has been isolated from a genomic library. The nucleotide sequence of the promoter and the 5'-coding region of this gene have been determined. A P. aeruginosa SOS consensus region, which functions as a binding site for the LexA repressor molecule, was not present in the 629-bp upstream region of the cloned uvrA gene. Analysis of transcriptional fusions with the lacZ gene demonstrates that, contrary to what happens with its homologous gene of E. coli, the P. aeruginosa uvrA gene is not DNA damage-inducible. However, the uvrA protein must be functional in P. aeruginosa cells because an uvrA-defective mutant is extremely sensitive to UV radiation.

KW - DNA damage

KW - Pseudomonas aeruginosa

KW - SOS system

KW - uvrA gene

U2 - https://doi.org/10.1016/S0027-5107(97)00061-4

DO - https://doi.org/10.1016/S0027-5107(97)00061-4

M3 - Article

VL - 377

SP - 149

EP - 155

JO - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

JF - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

SN - 0027-5107

ER -