Expression of the Bacillus thuringiensis mosquitocidal toxin Cry11Aa in the aquatic bacterium Asticcacaulis excentricus

Gemma Armengol, Oscar Enrique Guevara, Sergio Orduz, Neil Crickmore

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

A mosquitocidal aquatic bacterium has been developed by introducing an operon containing the cry11Aa, and p20 genes from Bacillus thuringiensis subsp. israelensis (Bti) into the gram-negative aquatic bacterium Asticcacaulis excentricus. After transformation, the cry11Aa gene was successfully expressed in recombinant A. excentricus under the tac promoter, at the level of 0.04 pg/cell. The recombinant bacteria were toxic to Aedes aegypti larvae with an LC50 of 6.83 × 105 cells/mL. We believe that these bacteria may have potential as genetically engineered microorganisms for the control of mosquito larvae. © Springer Science+Business Media, Inc. 2005.
Original languageEnglish
Pages (from-to)430-433
JournalCurrent Microbiology
Volume51
DOIs
Publication statusPublished - 1 Dec 2005

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