Expression of a green fluorescence protein-carrier protein into mouse spermatozoa

Josep M. Fernández-Novell, Roger R. Gomis, Joan Ballester, Joan J. Guinovart, Joan C. Ferrer, Juan Enrique Rodriguez Gil, Teresa Josefa Rigau Mas, Maria Dels Dolors Izquierdo Tugas, Pedro Jose Otaegui Goya, Maria Jesus Palomo Peiro, Maria Teresa Mogas Amoros

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Intra-testicular inoculation of an adenoviral vector carrying the fusion gene Aequorea victoria green fluorescence protein/rat-liver glycogen synthase (GFP/LGS) resulted in the presence of GFP/GLS in spermatozoa from 7 days to, at least, 16 days after inoculation. The GFP/LGS was detected in the sperm heads after an "in vitro" fertilization procedure, either before or after the oocyte penetration. Our results indicate that spermatozoa carrying GFP/LGS protein conserved their fertilizing ability and were also detectable after oocyte penetration. This technique will allow to develop an easy system to follow the fate of mature sperm proteins. © 2002 Elsevier Science (USA). All rights reserved.
Original languageEnglish
Pages (from-to)841-846
JournalBiochemical and Biophysical Research Communications
Volume297
DOIs
Publication statusPublished - 7 Nov 2002

Keywords

  • Green fluorescence protein
  • Mature spermatozoa
  • Protein tracking

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