Expression of a green fluorescence protein-carrier protein into mouse spermatozoa

Josep M. Fernández-Novell; Roger R. Gomis; Joan Ballester; Joan J. Guinovart; Joan C. Ferrer; Juan Enrique Rodriguez Gil; Teresa Josefa Rigau Mas; Maria Dels Dolors Izquierdo Tugas; Pedro Jose Otaegui Goya; Maria Jesus Palomo Peiro; Maria Teresa Mogas Amoros

doi:https://doi.org/10.1016/S0006-291X(02)02295-7

Expression of a green fluorescence protein-carrier protein into mouse spermatozoa

Josep M. Fernández-Novell, Roger R. Gomis, Joan Ballester, Joan J. Guinovart, Joan C. Ferrer, Juan Enrique Rodriguez Gil, Teresa Josefa Rigau Mas, Maria Dels Dolors Izquierdo Tugas, Pedro Jose Otaegui Goya, Maria Jesus Palomo Peiro, Maria Teresa Mogas Amoros

Research output: Contribution to journal › Article › Research › peer-review

Abstract

Intra-testicular inoculation of an adenoviral vector carrying the fusion gene Aequorea victoria green fluorescence protein/rat-liver glycogen synthase (GFP/LGS) resulted in the presence of GFP/GLS in spermatozoa from 7 days to, at least, 16 days after inoculation. The GFP/LGS was detected in the sperm heads after an "in vitro" fertilization procedure, either before or after the oocyte penetration. Our results indicate that spermatozoa carrying GFP/LGS protein conserved their fertilizing ability and were also detectable after oocyte penetration. This technique will allow to develop an easy system to follow the fate of mature sperm proteins. © 2002 Elsevier Science (USA). All rights reserved.

Original language	English
Pages (from-to)	841-846
Journal	Biochemical and Biophysical Research Communications
Volume	297
DOIs	https://doi.org/10.1016/S0006-291X(02)02295-7
Publication status	Published - 7 Nov 2002

Keywords

Green fluorescence protein
Mature spermatozoa
Protein tracking

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Fernández-Novell, J. M., Gomis, R. R., Ballester, J., Guinovart, J. J., Ferrer, J. C., Rodriguez Gil, J. E., Rigau Mas, T. J., Izquierdo Tugas, M. D. D., Otaegui Goya, P. J., Palomo Peiro, M. J., & Mogas Amoros, M. T. (2002). Expression of a green fluorescence protein-carrier protein into mouse spermatozoa. Biochemical and Biophysical Research Communications, 297, 841-846. https://doi.org/10.1016/S0006-291X(02)02295-7

Fernández-Novell, Josep M. ; Gomis, Roger R. ; Ballester, Joan ; Guinovart, Joan J. ; Ferrer, Joan C. ; Rodriguez Gil, Juan Enrique ; Rigau Mas, Teresa Josefa ; Izquierdo Tugas, Maria Dels Dolors ; Otaegui Goya, Pedro Jose ; Palomo Peiro, Maria Jesus ; Mogas Amoros, Maria Teresa. / Expression of a green fluorescence protein-carrier protein into mouse spermatozoa. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 297. pp. 841-846.

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title = "Expression of a green fluorescence protein-carrier protein into mouse spermatozoa",

abstract = "Intra-testicular inoculation of an adenoviral vector carrying the fusion gene Aequorea victoria green fluorescence protein/rat-liver glycogen synthase (GFP/LGS) resulted in the presence of GFP/GLS in spermatozoa from 7 days to, at least, 16 days after inoculation. The GFP/LGS was detected in the sperm heads after an {"}in vitro{"} fertilization procedure, either before or after the oocyte penetration. Our results indicate that spermatozoa carrying GFP/LGS protein conserved their fertilizing ability and were also detectable after oocyte penetration. This technique will allow to develop an easy system to follow the fate of mature sperm proteins. {\textcopyright} 2002 Elsevier Science (USA). All rights reserved.",

keywords = "Green fluorescence protein, Mature spermatozoa, Protein tracking",

author = "Fern{\'a}ndez-Novell, {Josep M.} and Gomis, {Roger R.} and Joan Ballester and Guinovart, {Joan J.} and Ferrer, {Joan C.} and {Rodriguez Gil}, {Juan Enrique} and {Rigau Mas}, {Teresa Josefa} and {Izquierdo Tugas}, {Maria Dels Dolors} and {Otaegui Goya}, {Pedro Jose} and {Palomo Peiro}, {Maria Jesus} and {Mogas Amoros}, {Maria Teresa}",

year = "2002",

month = nov,

day = "7",

doi = "https://doi.org/10.1016/S0006-291X(02)02295-7",

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Fernández-Novell, JM, Gomis, RR, Ballester, J, Guinovart, JJ, Ferrer, JC, Rodriguez Gil, JE , Rigau Mas, TJ , Izquierdo Tugas, MDD, Otaegui Goya, PJ, Palomo Peiro, MJ & Mogas Amoros, MT 2002, 'Expression of a green fluorescence protein-carrier protein into mouse spermatozoa', Biochemical and Biophysical Research Communications, vol. 297, pp. 841-846. https://doi.org/10.1016/S0006-291X(02)02295-7

Expression of a green fluorescence protein-carrier protein into mouse spermatozoa. / Fernández-Novell, Josep M.; Gomis, Roger R.; Ballester, Joan; Guinovart, Joan J.; Ferrer, Joan C.; Rodriguez Gil, Juan Enrique ; Rigau Mas, Teresa Josefa ; Izquierdo Tugas, Maria Dels Dolors; Otaegui Goya, Pedro Jose; Palomo Peiro, Maria Jesus ; Mogas Amoros, Maria Teresa.

In: Biochemical and Biophysical Research Communications, Vol. 297, 07.11.2002, p. 841-846.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Expression of a green fluorescence protein-carrier protein into mouse spermatozoa

AU - Fernández-Novell, Josep M.

AU - Gomis, Roger R.

AU - Ballester, Joan

AU - Guinovart, Joan J.

AU - Ferrer, Joan C.

AU - Rodriguez Gil, Juan Enrique

AU - Rigau Mas, Teresa Josefa

AU - Izquierdo Tugas, Maria Dels Dolors

AU - Otaegui Goya, Pedro Jose

AU - Palomo Peiro, Maria Jesus

AU - Mogas Amoros, Maria Teresa

PY - 2002/11/7

Y1 - 2002/11/7

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AB - Intra-testicular inoculation of an adenoviral vector carrying the fusion gene Aequorea victoria green fluorescence protein/rat-liver glycogen synthase (GFP/LGS) resulted in the presence of GFP/GLS in spermatozoa from 7 days to, at least, 16 days after inoculation. The GFP/LGS was detected in the sperm heads after an "in vitro" fertilization procedure, either before or after the oocyte penetration. Our results indicate that spermatozoa carrying GFP/LGS protein conserved their fertilizing ability and were also detectable after oocyte penetration. This technique will allow to develop an easy system to follow the fate of mature sperm proteins. © 2002 Elsevier Science (USA). All rights reserved.

KW - Green fluorescence protein

KW - Mature spermatozoa

KW - Protein tracking

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