TY - JOUR
T1 - Expression of a green fluorescence protein-carrier protein into mouse spermatozoa
AU - Fernández-Novell, Josep M.
AU - Gomis, Roger R.
AU - Ballester, Joan
AU - Guinovart, Joan J.
AU - Ferrer, Joan C.
AU - Rodriguez Gil, Juan Enrique
AU - Rigau Mas, Teresa Josefa
AU - Izquierdo Tugas, Maria Dels Dolors
AU - Otaegui Goya, Pedro Jose
AU - Palomo Peiro, Maria Jesus
AU - Mogas Amoros, Maria Teresa
PY - 2002/11/7
Y1 - 2002/11/7
N2 - Intra-testicular inoculation of an adenoviral vector carrying the fusion gene Aequorea victoria green fluorescence protein/rat-liver glycogen synthase (GFP/LGS) resulted in the presence of GFP/GLS in spermatozoa from 7 days to, at least, 16 days after inoculation. The GFP/LGS was detected in the sperm heads after an "in vitro" fertilization procedure, either before or after the oocyte penetration. Our results indicate that spermatozoa carrying GFP/LGS protein conserved their fertilizing ability and were also detectable after oocyte penetration. This technique will allow to develop an easy system to follow the fate of mature sperm proteins. © 2002 Elsevier Science (USA). All rights reserved.
AB - Intra-testicular inoculation of an adenoviral vector carrying the fusion gene Aequorea victoria green fluorescence protein/rat-liver glycogen synthase (GFP/LGS) resulted in the presence of GFP/GLS in spermatozoa from 7 days to, at least, 16 days after inoculation. The GFP/LGS was detected in the sperm heads after an "in vitro" fertilization procedure, either before or after the oocyte penetration. Our results indicate that spermatozoa carrying GFP/LGS protein conserved their fertilizing ability and were also detectable after oocyte penetration. This technique will allow to develop an easy system to follow the fate of mature sperm proteins. © 2002 Elsevier Science (USA). All rights reserved.
KW - Green fluorescence protein
KW - Mature spermatozoa
KW - Protein tracking
U2 - https://doi.org/10.1016/S0006-291X(02)02295-7
DO - https://doi.org/10.1016/S0006-291X(02)02295-7
M3 - Article
VL - 297
SP - 841
EP - 846
ER -