Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos

Erika Alina Ordóñez-León; Iris Martínez-Rodero; Tania García-Martínez; Manel López-Béjar; Marc Yeste; Elena Mercade; Teresa Mogas

doi:10.3390/ijms23137069

Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos

Erika Alina Ordóñez-León, Iris Martínez-Rodero, Tania García-Martínez, Manel López-Béjar, Marc Yeste, Elena Mercade, Teresa Mogas^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

2 Citations (Scopus)

Abstract

This study aimed to assess the cryoprotectant role of exopolysaccharide (EPS) ID1, produced by Antarctic Pseudomonas sp., in the vitrification of in vitro-produced (IVP) bovine embryos. IVP day 7 (D7) and day 8 (D8) expanded blastocysts derived from cow or calf oocytes were vitrified without supplementation (EPS0) or supplemented with 10 mu g/mL (EPS10) or 100 mu g/mL (EPS100) EPS ID1. The effect of EPS ID1 was assessed in post-warming re-expansion and hatching rates, differential cell count, apoptosis rate, and gene expression. EPS100 re-expansion rates were significantly higher than those observed for the EPS0 and EPS10 treatments, regardless of culture length or oocyte source. EPS100 hatching rate was similar to the one of the fresh blastocysts except for those D7 blastocysts derived from calf oocytes. No differences were observed among EPS ID1 treatments when the inner cell mass, trophectoderm, and total cell number were assessed. Although apoptosis rates were higher (p

Original language	English
Article number	7069
Number of pages	18
Journal	International journal of molecular sciences
Volume	23
Issue number	13
DOIs	https://doi.org/10.3390/ijms23137069
Publication status	Published - Jul 2022

Keywords

TUNEL
blastocyst
cryopreservation
embryo development
gene expression regulation
inner cell mass
total cell number
Vitrification
Blastocyst
Fertilization in Vitro/methods
Cryopreservation/methods
bcl-2-Associated X Protein/genetics
Animals
Cattle
Embryo Culture Techniques/methods
Female
SURVIVAL
HYPOTHERMIC STORAGE
IN-VITRO
PROTEIN TYPE-III
BLASTOCYSTS
ANTIFREEZE PROTEINS
MATURE MOUSE OOCYTES
CULTURE
BACTERIUM
EXPRESSION

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title = "Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos",

abstract = "This study aimed to assess the cryoprotectant role of exopolysaccharide (EPS) ID1, produced by Antarctic Pseudomonas sp., in the vitrification of in vitro-produced (IVP) bovine embryos. IVP day 7 (D7) and day 8 (D8) expanded blastocysts derived from cow or calf oocytes were vitrified without supplementation (EPS0) or supplemented with 10 mu g/mL (EPS10) or 100 mu g/mL (EPS100) EPS ID1. The effect of EPS ID1 was assessed in post-warming re-expansion and hatching rates, differential cell count, apoptosis rate, and gene expression. EPS100 re-expansion rates were significantly higher than those observed for the EPS0 and EPS10 treatments, regardless of culture length or oocyte source. EPS100 hatching rate was similar to the one of the fresh blastocysts except for those D7 blastocysts derived from calf oocytes. No differences were observed among EPS ID1 treatments when the inner cell mass, trophectoderm, and total cell number were assessed. Although apoptosis rates were higher (p",

keywords = "TUNEL, blastocyst, cryopreservation, embryo development, gene expression regulation, inner cell mass, total cell number, Vitrification, Blastocyst, Fertilization in Vitro/methods, Cryopreservation/methods, bcl-2-Associated X Protein/genetics, Animals, Cattle, Embryo Culture Techniques/methods, Female, SURVIVAL, HYPOTHERMIC STORAGE, IN-VITRO, PROTEIN TYPE-III, BLASTOCYSTS, ANTIFREEZE PROTEINS, MATURE MOUSE OOCYTES, CULTURE, BACTERIUM, EXPRESSION",

author = "Ord{\'o}{\~n}ez-Le{\'o}n, {Erika Alina} and Iris Mart{\'i}nez-Rodero and Tania Garc{\'i}a-Mart{\'i}nez and Manel L{\'o}pez-B{\'e}jar and Marc Yeste and Elena Mercade and Teresa Mogas",

note = "Funding Information: This study was supported by the Spanish Ministry of Science and Innovation (Project PID2020-116531RB-I00) and Generalitat de Catalunya (Project No. 2017 SGR 1229). Alina Ord{\'o}{\~n}ez-Le{\'o}n was awarded a post-doctoral scholarship from the Mexican National Conseil for Science and Technology (CONACYT-45193). Iris Mart{\'i}nez-Rodero was awarded a scholarship from the Spanish Ministry of Science, Innovation, and Universities (BES-2017-081962). Funding Information: Funding: This study was supported by the Spanish Ministry of Science and Innovation (Project PID2020-116531RB-I00) and Generalitat de Catalunya (Project No. 2017 SGR 1229). Alina Ord{\'o}{\~n}ez-Le{\'o}n was awarded a post-doctoral scholarship from the Mexican National Conseil for Science and Technology (CONACYT-45193). Iris Mart{\'i}nez-Rodero was awarded a scholarship from the Spanish Ministry of Science, Innovation, and Universities (BES-2017-081962). Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2022",

month = jul,

doi = "10.3390/ijms23137069",

language = "English",

volume = "23",

journal = "International Journal of Molecular Sciences",

issn = "1661-6596",

number = "13",

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TY - JOUR

T1 - Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos

AU - Ordóñez-León, Erika Alina

AU - Martínez-Rodero, Iris

AU - García-Martínez, Tania

AU - López-Béjar, Manel

AU - Yeste, Marc

AU - Mercade, Elena

AU - Mogas, Teresa

N1 - Funding Information: This study was supported by the Spanish Ministry of Science and Innovation (Project PID2020-116531RB-I00) and Generalitat de Catalunya (Project No. 2017 SGR 1229). Alina Ordóñez-León was awarded a post-doctoral scholarship from the Mexican National Conseil for Science and Technology (CONACYT-45193). Iris Martínez-Rodero was awarded a scholarship from the Spanish Ministry of Science, Innovation, and Universities (BES-2017-081962). Funding Information: Funding: This study was supported by the Spanish Ministry of Science and Innovation (Project PID2020-116531RB-I00) and Generalitat de Catalunya (Project No. 2017 SGR 1229). Alina Ordóñez-León was awarded a post-doctoral scholarship from the Mexican National Conseil for Science and Technology (CONACYT-45193). Iris Martínez-Rodero was awarded a scholarship from the Spanish Ministry of Science, Innovation, and Universities (BES-2017-081962). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2022/7

Y1 - 2022/7

N2 - This study aimed to assess the cryoprotectant role of exopolysaccharide (EPS) ID1, produced by Antarctic Pseudomonas sp., in the vitrification of in vitro-produced (IVP) bovine embryos. IVP day 7 (D7) and day 8 (D8) expanded blastocysts derived from cow or calf oocytes were vitrified without supplementation (EPS0) or supplemented with 10 mu g/mL (EPS10) or 100 mu g/mL (EPS100) EPS ID1. The effect of EPS ID1 was assessed in post-warming re-expansion and hatching rates, differential cell count, apoptosis rate, and gene expression. EPS100 re-expansion rates were significantly higher than those observed for the EPS0 and EPS10 treatments, regardless of culture length or oocyte source. EPS100 hatching rate was similar to the one of the fresh blastocysts except for those D7 blastocysts derived from calf oocytes. No differences were observed among EPS ID1 treatments when the inner cell mass, trophectoderm, and total cell number were assessed. Although apoptosis rates were higher (p

AB - This study aimed to assess the cryoprotectant role of exopolysaccharide (EPS) ID1, produced by Antarctic Pseudomonas sp., in the vitrification of in vitro-produced (IVP) bovine embryos. IVP day 7 (D7) and day 8 (D8) expanded blastocysts derived from cow or calf oocytes were vitrified without supplementation (EPS0) or supplemented with 10 mu g/mL (EPS10) or 100 mu g/mL (EPS100) EPS ID1. The effect of EPS ID1 was assessed in post-warming re-expansion and hatching rates, differential cell count, apoptosis rate, and gene expression. EPS100 re-expansion rates were significantly higher than those observed for the EPS0 and EPS10 treatments, regardless of culture length or oocyte source. EPS100 hatching rate was similar to the one of the fresh blastocysts except for those D7 blastocysts derived from calf oocytes. No differences were observed among EPS ID1 treatments when the inner cell mass, trophectoderm, and total cell number were assessed. Although apoptosis rates were higher (p

KW - TUNEL

KW - blastocyst

KW - cryopreservation

KW - embryo development

KW - gene expression regulation

KW - inner cell mass

KW - total cell number

KW - Vitrification

KW - Blastocyst

KW - Fertilization in Vitro/methods

KW - Cryopreservation/methods

KW - bcl-2-Associated X Protein/genetics

KW - Animals

KW - Cattle

KW - Embryo Culture Techniques/methods

KW - Female

KW - SURVIVAL

KW - HYPOTHERMIC STORAGE

KW - IN-VITRO

KW - PROTEIN TYPE-III

KW - BLASTOCYSTS

KW - ANTIFREEZE PROTEINS

KW - MATURE MOUSE OOCYTES

KW - CULTURE

KW - BACTERIUM

KW - EXPRESSION

UR - http://www.scopus.com/inward/record.url?scp=85132748245&partnerID=8YFLogxK

U2 - 10.3390/ijms23137069

DO - 10.3390/ijms23137069

M3 - Article

C2 - 35806071

AN - SCOPUS:85132748245

VL - 23

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 13

M1 - 7069

ER -

Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos

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Keywords

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