Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos

Erika Alina Ordóñez-León, Iris Martínez-Rodero, Tania García-Martínez, Manel López-Béjar, Marc Yeste, Elena Mercade, Teresa Mogas*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

This study aimed to assess the cryoprotectant role of exopolysaccharide (EPS) ID1, produced by Antarctic Pseudomonas sp., in the vitrification of in vitro-produced (IVP) bovine embryos. IVP day 7 (D7) and day 8 (D8) expanded blastocysts derived from cow or calf oocytes were vitrified without supplementation (EPS0) or supplemented with 10 mu g/mL (EPS10) or 100 mu g/mL (EPS100) EPS ID1. The effect of EPS ID1 was assessed in post-warming re-expansion and hatching rates, differential cell count, apoptosis rate, and gene expression. EPS100 re-expansion rates were significantly higher than those observed for the EPS0 and EPS10 treatments, regardless of culture length or oocyte source. EPS100 hatching rate was similar to the one of the fresh blastocysts except for those D7 blastocysts derived from calf oocytes. No differences were observed among EPS ID1 treatments when the inner cell mass, trophectoderm, and total cell number were assessed. Although apoptosis rates were higher (p

Original languageEnglish
Article number7069
Number of pages18
JournalInternational journal of molecular sciences
Volume23
Issue number13
DOIs
Publication statusPublished - Jul 2022

Keywords

  • TUNEL
  • blastocyst
  • cryopreservation
  • embryo development
  • gene expression regulation
  • inner cell mass
  • total cell number
  • Vitrification
  • Blastocyst
  • Fertilization in Vitro/methods
  • Cryopreservation/methods
  • bcl-2-Associated X Protein/genetics
  • Animals
  • Cattle
  • Embryo Culture Techniques/methods
  • Female
  • SURVIVAL
  • HYPOTHERMIC STORAGE
  • IN-VITRO
  • PROTEIN TYPE-III
  • BLASTOCYSTS
  • ANTIFREEZE PROTEINS
  • MATURE MOUSE OOCYTES
  • CULTURE
  • BACTERIUM
  • EXPRESSION

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