TY - JOUR
T1 - Exonization of Alu-generated Splice Variants in the Survivin Gene of Human and Non-human Primates
AU - Mola, Gemma
AU - Vela, Elena
AU - Fernández-Figueras, Ma Teresa
AU - Isamat, Marcos
AU - Muñoz-Mármol, Ana M.
PY - 2007/3/2
Y1 - 2007/3/2
N2 - Survivin is a member of the inhibitor apoptosis family that is overexpressed in many malignancies. It has five known alternative splice forms, some of which differ in their antiapoptotic properties and expression levels in human cancers. Here we describe a novel donor splice site (DSS), 2B + 32 DSS, which is used in conjunction with survivin alternative exon 2B, resulting in the inclusion of 32 additional nucleotides from intron 2 at the 3′ end of this exon. Sequence analysis showed that both the classical exon 2B DSS and 2B + 32 are provided by an Alu sequence, which is inserted in intron 2 downstream of a functional acceptor splice site, leading to the exonization of part of the repetitive element. Minor transcripts including the 2B + 32 alternative exon, or retaining the whole intronic region comprised between exons 2B and 3, were detected in several human cell lines and in some human tissues. Survivin 2B + 32 containing variants acquire a premature stop codon (PTC) and may therefore be degraded by the nonsense mediated decay pathway. The implication of these novel isoforms, as well as other PTC+ survivin variants, in the overall regulation of survivin expression is discussed. Sequence analysis of intron 2 which contains the Alu Y element was performed on different primate species in order to trace its insertion and exonization during primate evolution. © 2006 Elsevier Ltd. All rights reserved.
AB - Survivin is a member of the inhibitor apoptosis family that is overexpressed in many malignancies. It has five known alternative splice forms, some of which differ in their antiapoptotic properties and expression levels in human cancers. Here we describe a novel donor splice site (DSS), 2B + 32 DSS, which is used in conjunction with survivin alternative exon 2B, resulting in the inclusion of 32 additional nucleotides from intron 2 at the 3′ end of this exon. Sequence analysis showed that both the classical exon 2B DSS and 2B + 32 are provided by an Alu sequence, which is inserted in intron 2 downstream of a functional acceptor splice site, leading to the exonization of part of the repetitive element. Minor transcripts including the 2B + 32 alternative exon, or retaining the whole intronic region comprised between exons 2B and 3, were detected in several human cell lines and in some human tissues. Survivin 2B + 32 containing variants acquire a premature stop codon (PTC) and may therefore be degraded by the nonsense mediated decay pathway. The implication of these novel isoforms, as well as other PTC+ survivin variants, in the overall regulation of survivin expression is discussed. Sequence analysis of intron 2 which contains the Alu Y element was performed on different primate species in order to trace its insertion and exonization during primate evolution. © 2006 Elsevier Ltd. All rights reserved.
KW - alternative splicing
KW - Alu-exonization
KW - nonsense mediated decay
KW - premature termination codon
KW - survivin
U2 - 10.1016/j.jmb.2006.11.089
DO - 10.1016/j.jmb.2006.11.089
M3 - Article
VL - 366
SP - 1055
EP - 1063
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
SN - 0022-2836
IS - 4
ER -