Excretion of BSE and scrapie prions in stools from murine models

Carlos Maluquer de Motes, Jacques Grassi, Stephanie Simon, Maria Eugenia Herva, Juan Maria Torres, Marti Pumarola, Rosina Girones*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Abstract

Faeces from infected animals have been suggested as a potential source of contamination and transmission of prion diseases in the environment. This work describes the development of a procedure for the detection of PrPres in stools which is based on a detergent-based extraction and immunoprecipitation (IP). The procedure was evaluated by analyzing TSE-spiked sheep and mice faeces, and proved to be specific for PrPres with sensitivities of 5-10 μg of infected brain tissue. In order to analyze the shedding of prions, we studied stools from orally inoculated mice over 4-days post-inoculation and also stools from terminally sick scrapie-infected mice. PrPres was only detected in stools shortly after the oral ingestion of TSE agents. The procedure described could be a useful tool for studying the excretion of prions and for evaluating potential environmental contamination by prions.

Original languageAmerican English
Pages (from-to)205-211
Number of pages7
JournalVeterinary Microbiology
Volume131
Issue number1-2
DOIs
Publication statusPublished - 18 Sep 2008

Keywords

  • BSE
  • Detection
  • Prion
  • Scrapie
  • Stool

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