Excretion of BSE and scrapie prions in stools from murine models

Marti Pumarola Batlle; Carlos Maluquer de Motes; Jacques Grassi; Stephanie Simon; Maria Eugenia Herva; Juan Maria Torres; Rosina Girones

doi:https://doi.org/10.1016/j.vetmic.2008.02.014

Excretion of BSE and scrapie prions in stools from murine models

Marti Pumarola Batlle, Carlos Maluquer de Motes, Jacques Grassi, Stephanie Simon, Maria Eugenia Herva, Juan Maria Torres, Rosina Girones

Department of Animal Medicine and Surgery

Research output: Contribution to journal › Article › Research › peer-review

11 Citations (Scopus)

Abstract

Faeces from infected animals have been suggested as a potential source of contamination and transmission of prion diseases in the environment. This work describes the development of a procedure for the detection of PrPres in stools which is based on a detergent-based extraction and immunoprecipitation (IP). The procedure was evaluated by analyzing TSE-spiked sheep and mice faeces, and proved to be specific for PrPres with sensitivities of 5-10 μg of infected brain tissue. In order to analyze the shedding of prions, we studied stools from orally inoculated mice over 4-days post-inoculation and also stools from terminally sick scrapie-infected mice. PrPres was only detected in stools shortly after the oral ingestion of TSE agents. The procedure described could be a useful tool for studying the excretion of prions and for evaluating potential environmental contamination by prions. © 2008 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	205-211
Journal	Veterinary Microbiology
Volume	131
DOIs	https://doi.org/10.1016/j.vetmic.2008.02.014
Publication status	Published - 18 Sep 2008

Keywords

BSE
Detection
Prion
Scrapie
Stool

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@article{d3927b29807a436da4ed4b3365a5ecab,

title = "Excretion of BSE and scrapie prions in stools from murine models",

abstract = "Faeces from infected animals have been suggested as a potential source of contamination and transmission of prion diseases in the environment. This work describes the development of a procedure for the detection of PrPres in stools which is based on a detergent-based extraction and immunoprecipitation (IP). The procedure was evaluated by analyzing TSE-spiked sheep and mice faeces, and proved to be specific for PrPres with sensitivities of 5-10 μg of infected brain tissue. In order to analyze the shedding of prions, we studied stools from orally inoculated mice over 4-days post-inoculation and also stools from terminally sick scrapie-infected mice. PrPres was only detected in stools shortly after the oral ingestion of TSE agents. The procedure described could be a useful tool for studying the excretion of prions and for evaluating potential environmental contamination by prions. {\textcopyright} 2008 Elsevier B.V. All rights reserved.",

keywords = "BSE, Detection, Prion, Scrapie, Stool",

author = "{Pumarola Batlle}, Marti and {Maluquer de Motes}, Carlos and Jacques Grassi and Stephanie Simon and Herva, {Maria Eugenia} and Torres, {Juan Maria} and Rosina Girones",

year = "2008",

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doi = "https://doi.org/10.1016/j.vetmic.2008.02.014",

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TY - JOUR

T1 - Excretion of BSE and scrapie prions in stools from murine models

AU - Pumarola Batlle, Marti

AU - Maluquer de Motes, Carlos

AU - Grassi, Jacques

AU - Simon, Stephanie

AU - Herva, Maria Eugenia

AU - Torres, Juan Maria

AU - Girones, Rosina

PY - 2008/9/18

Y1 - 2008/9/18

N2 - Faeces from infected animals have been suggested as a potential source of contamination and transmission of prion diseases in the environment. This work describes the development of a procedure for the detection of PrPres in stools which is based on a detergent-based extraction and immunoprecipitation (IP). The procedure was evaluated by analyzing TSE-spiked sheep and mice faeces, and proved to be specific for PrPres with sensitivities of 5-10 μg of infected brain tissue. In order to analyze the shedding of prions, we studied stools from orally inoculated mice over 4-days post-inoculation and also stools from terminally sick scrapie-infected mice. PrPres was only detected in stools shortly after the oral ingestion of TSE agents. The procedure described could be a useful tool for studying the excretion of prions and for evaluating potential environmental contamination by prions. © 2008 Elsevier B.V. All rights reserved.

AB - Faeces from infected animals have been suggested as a potential source of contamination and transmission of prion diseases in the environment. This work describes the development of a procedure for the detection of PrPres in stools which is based on a detergent-based extraction and immunoprecipitation (IP). The procedure was evaluated by analyzing TSE-spiked sheep and mice faeces, and proved to be specific for PrPres with sensitivities of 5-10 μg of infected brain tissue. In order to analyze the shedding of prions, we studied stools from orally inoculated mice over 4-days post-inoculation and also stools from terminally sick scrapie-infected mice. PrPres was only detected in stools shortly after the oral ingestion of TSE agents. The procedure described could be a useful tool for studying the excretion of prions and for evaluating potential environmental contamination by prions. © 2008 Elsevier B.V. All rights reserved.

KW - BSE

KW - Detection

KW - Prion

KW - Scrapie

KW - Stool

U2 - https://doi.org/10.1016/j.vetmic.2008.02.014

DO - https://doi.org/10.1016/j.vetmic.2008.02.014

M3 - Article

VL - 131

SP - 205

EP - 211

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

ER -