TY - JOUR
T1 - Examination of various biomarkers measuring genotoxic endpoints from Barcelona airport personnel
AU - Pitarque, M.
AU - Creus, A.
AU - Marcos, R.
AU - Hughes, J. A.
AU - Anderson, D.
N1 - Funding Information:
This investigation was supported in part by the European Union (EU, EV5V-CT92-0221), the Spanish Ministry of Education and Science (CICYT, SAF95-0813) and the Generalitat de Catalunya (CIRIT, SGR95-00512). We are grateful to the Universitat Autònoma de Barcelona for supporting M. Pitarque's stay at BIBRA International. We would like to thank T. Amador, A. Corral, and G. Umbert for their expert technical assistance in the processing and scoring of the samples. The collaboration of the Centre de Seguretat i Condicions de Salut en el Treball (Departament de Treball, Generalitat de Catalunya) in gas chromatography analysis of workplace air samples is very much appreciated.
PY - 1999/4/6
Y1 - 1999/4/6
N2 - Three different biomarkers: sister-chromatid exchanges (SCE), micronuclei (MN), and the Comet assay, were used to evaluate different kinds of genetic damage in peripheral blood lymphocytes from 34 male workers at Barcelona airport, exposed to low levels of hydrocarbons and jet fuel derivatives. The control group consisted of 11 unexposed men. We also investigated the ras p21 protein levels in plasma, in order to evaluate whether the ras gene could serve as a suitable potential marker of carcinogenic pollution in occupationally exposed cohorts. SCE and MN analyses failed to detect any statistically significant increase in the airport workers when compared with the controls, and in fact, the frequency of binucleated cells with MN in the exposed group was significantly lower than that obtained in the control. However, slight but significant differences in the mean comet length and genetic damage index were observed between the exposed and control groups when using the Comet assay. There were no statistically significant differences between both groups in p21 plasma levels. Smoking was shown to affect significantly both SCE and high frequency cells (HFC) in the exposed group. Copyright (C) 1999 Elsevier Science B.V.
AB - Three different biomarkers: sister-chromatid exchanges (SCE), micronuclei (MN), and the Comet assay, were used to evaluate different kinds of genetic damage in peripheral blood lymphocytes from 34 male workers at Barcelona airport, exposed to low levels of hydrocarbons and jet fuel derivatives. The control group consisted of 11 unexposed men. We also investigated the ras p21 protein levels in plasma, in order to evaluate whether the ras gene could serve as a suitable potential marker of carcinogenic pollution in occupationally exposed cohorts. SCE and MN analyses failed to detect any statistically significant increase in the airport workers when compared with the controls, and in fact, the frequency of binucleated cells with MN in the exposed group was significantly lower than that obtained in the control. However, slight but significant differences in the mean comet length and genetic damage index were observed between the exposed and control groups when using the Comet assay. There were no statistically significant differences between both groups in p21 plasma levels. Smoking was shown to affect significantly both SCE and high frequency cells (HFC) in the exposed group. Copyright (C) 1999 Elsevier Science B.V.
KW - Airport worker
KW - Comet assay
KW - Micronucleus
KW - Ras p21 protein
KW - Sister chromatid exchange
UR - http://www.scopus.com/inward/record.url?scp=0032946069&partnerID=8YFLogxK
U2 - 10.1016/S1383-5718(99)00026-1
DO - 10.1016/S1383-5718(99)00026-1
M3 - Article
C2 - 10209343
AN - SCOPUS:0032946069
SN - 1383-5718
VL - 440
SP - 195
EP - 204
JO - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
JF - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
IS - 2
ER -