Evidence that mobile lipids detected in rat brain glioma by 1H nuclear magnetic resonance correspond to lipid droplets

Chantal Rémy, Nathalie Fouilhé, Ignasi Barba, Ernest Sam-Laï, Hana Lahrech, Maria Gracia Cucurella, Marguerite Izquierdo, Angel Moreno, Anne Ziegler, Raphael Massarelli, Michel Décorps, Caries Arús

Research output: Contribution to journalArticleResearchpeer-review

128 Citations (Scopus)

Abstract

Mobile lipids have been detected by proton nuclear magnetic resonance (NMR) in animal and human tumors (cultured cells, biopsies, and in vivo), but their origin and subcellular location are still unclear. They have been associated with malignancy, metastatic ability, drug resistance, and necrosis. We wanted to determine whether these lipids are located within plasma membrane microdomains or in lipid droplets for a C6 cell-induced rat glioma. NMR-visible mobile lipids were found in all subcellular fractions isolated from the rat tumor, except in the cytosolic supernatants. Transmission electron microscopy showed that lipid droplets were present in all subcellular fractions containing NMR-visible lipids and in the necrotic and perinecrotic areas of the tumor. The mean diameter of droplets isolated by flotation in the subcellular fractionation protocol was 0.97 μm (n = 682; droplet profile diameter range between 0.2 and 5.0 μm). The apparent diffusion coefficient for these lipids (46 ± 17 μm2s-1) measured in vivo by proton spectroscopy was four orders of magnitude higher than would be expected if mobile lipids were inside plasma membrane microdomains. The combined results demonstrated that mobile lipids detected in vivo by proton NMR in the C6 rat glioma are located in large lipid droplets, associated with the necrotic process.
Original languageEnglish
Pages (from-to)407-414
JournalCancer Research
Volume57
Issue number3
Publication statusPublished - 27 Feb 1997

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