Evidence supporting presence of two pacemakers in rat colon

L. Plujà, E. Albertí, E. Fernández, H. B. Mikkelsen, L. Thuneberg, M. Jiménez

Research output: Contribution to journalArticleResearchpeer-review

106 Citations (Scopus)

Abstract

Intracellular microelectrodes and organ bath techniques were used to study spontaneous cyclic electrical and mechanical activity in the rat colon. Electron microscopy and immunohistochemical studies showed two major populations of interstitial cells of Cajal (ICC): one associated with Auerbach's plexus (ICC-AP) and one with the submuscular plexus (ICC-SMP). The ICC-SMP network partly adhered to the submucosa when removed and was generally strongly damaged after separation of musculature and submucosa. Similarly, longitudinal muscle removal severely damaged AP. Two electrical and mechanical activity patterns were recorded: pattern A, low-frequency (0.5-1.5 cycles/min), high-amplitude oscillations; and pattern B, high-frequency (13-15 cycles/min), low-amplitude oscillations. Pattern A was recorded in preparations with intact AP but absent in those without intact AP. Pattern B was recorded in preparations with intact SMP but was absent in those lacking SMP. With full-thickness strips, the superimposed patterns A and B were recorded in circular muscle. When longitudinal muscle mechanical activity was recorded, only pattern A was present. We conclude that two pacemakers regulate rat colonic cyclic activity: the ICC-SMP network (responsible for cyclic slow waves and small-amplitude contractions) and the ICC-AP network (which may drive the cyclic depolarizations responsible for high-amplitude contractions). This is the first report showing consistent slow wave activity in the rodent colon.
Original languageEnglish
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume281
Issue number1 44-1
Publication statusPublished - 11 Oct 2001

Keywords

  • Interstitial cell of Cajal
  • Slow waves
  • Smooth muscle

Fingerprint Dive into the research topics of 'Evidence supporting presence of two pacemakers in rat colon'. Together they form a unique fingerprint.

Cite this