Evidence of agar gel electrophoresis changes of lipoprotein-x after phospholipase a and deoxycholic acid

J. C. Prison; M. R. Ras; J. Rubiés-Prat; S. Masdeu; S. Schwartz

doi:10.1159/000198074

Evidence of agar gel electrophoresis changes of lipoprotein-x after phospholipase a and deoxycholic acid

J. C. Prison, M. R. Ras, J. Rubiés-Prat, S. Masdeu, S. Schwartz

Department of Medicine

Research output: Contribution to journal › Article › Research › peer-review

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Abstract

The effect of phospholipase A from snake venom and deoxycholic acid on lipoprotein-X (LP-X) recovered from the cathode side of a previous agar gel electrophoresis is described. Adding phospholipase A and deoxycholic acid to the removed cathodal fraction is followed by a marked migration to the anode side on a second electrophoresis procedure. This seems to confirm that phospholipase A and bile salts act on LP-X particles modifying their agar gel electrophoretic migration characteristics. © 1977 S. Karger AG, Basel.

Original language	English
Pages (from-to)	199-202
Journal	Digestion
Volume	16
Issue number	1-2
DOIs	https://doi.org/10.1159/000198074
Publication status	Published - 1 Jan 1977

Keywords

Cholestasis
Deoxycholic acid
Electrophoresis
Lipoproteins
Liver disease
Phospholipase

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@article{a315a1039e834bf49aac348be53d484f,

title = "Evidence of agar gel electrophoresis changes of lipoprotein-x after phospholipase a and deoxycholic acid",

abstract = "The effect of phospholipase A from snake venom and deoxycholic acid on lipoprotein-X (LP-X) recovered from the cathode side of a previous agar gel electrophoresis is described. Adding phospholipase A and deoxycholic acid to the removed cathodal fraction is followed by a marked migration to the anode side on a second electrophoresis procedure. This seems to confirm that phospholipase A and bile salts act on LP-X particles modifying their agar gel electrophoretic migration characteristics. {\textcopyright} 1977 S. Karger AG, Basel.",

keywords = "Cholestasis, Deoxycholic acid, Electrophoresis, Lipoproteins, Liver disease, Phospholipase",

author = "Prison, {J. C.} and Ras, {M. R.} and J. Rubi{\'e}s-Prat and S. Masdeu and S. Schwartz",

year = "1977",

month = jan,

day = "1",

doi = "10.1159/000198074",

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volume = "16",

pages = "199--202",

journal = "Digestion",

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TY - JOUR

T1 - Evidence of agar gel electrophoresis changes of lipoprotein-x after phospholipase a and deoxycholic acid

AU - Prison, J. C.

AU - Ras, M. R.

AU - Rubiés-Prat, J.

AU - Masdeu, S.

AU - Schwartz, S.

PY - 1977/1/1

Y1 - 1977/1/1

N2 - The effect of phospholipase A from snake venom and deoxycholic acid on lipoprotein-X (LP-X) recovered from the cathode side of a previous agar gel electrophoresis is described. Adding phospholipase A and deoxycholic acid to the removed cathodal fraction is followed by a marked migration to the anode side on a second electrophoresis procedure. This seems to confirm that phospholipase A and bile salts act on LP-X particles modifying their agar gel electrophoretic migration characteristics. © 1977 S. Karger AG, Basel.

AB - The effect of phospholipase A from snake venom and deoxycholic acid on lipoprotein-X (LP-X) recovered from the cathode side of a previous agar gel electrophoresis is described. Adding phospholipase A and deoxycholic acid to the removed cathodal fraction is followed by a marked migration to the anode side on a second electrophoresis procedure. This seems to confirm that phospholipase A and bile salts act on LP-X particles modifying their agar gel electrophoretic migration characteristics. © 1977 S. Karger AG, Basel.

KW - Cholestasis

KW - Deoxycholic acid

KW - Electrophoresis

KW - Lipoproteins

KW - Liver disease

KW - Phospholipase

U2 - 10.1159/000198074

DO - 10.1159/000198074

M3 - Article

SN - 0012-2823

VL - 16

SP - 199

EP - 202

JO - Digestion

JF - Digestion

IS - 1-2

ER -

Evidence of agar gel electrophoresis changes of lipoprotein-x after phospholipase a and deoxycholic acid

Abstract

Keywords

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