Evidence for convergent evolution of CTX-M-14 ESBL in Escherichia coli and its prevalence

Ferran Navarro; Rául Jesús Mesa; Elisenda Miró; Laura Gómez; Beatriz Mirelis; Pere Coll

doi:10.1111/j.1574-6968.2007.00791.x

Evidence for convergent evolution of CTX-M-14 ESBL in Escherichia coli and its prevalence

Ferran Navarro, Rául Jesús Mesa, Elisenda Miró, Laura Gómez, Beatriz Mirelis, Pere Coll

Department of Genetics and Microbiology

Research output: Contribution to journal › Article › Research › peer-review

24 Citations (Scopus)

Abstract

A total of 2440 Escherichia coli strains isolated in 2003 at the Hospital de la Santa Creu i Sant Pau were evaluated for the presence of extended-spectrum β-lactamases. Two different nucleotide sequences that encode the same β-lactamase, CTX-M-14, were detected when the blaCTX-M-14-genes of 35 E. coli isolates were analysed. Thirty-two of the 35 had the previously described sequence of the blaCTX-M-14 (AF252622), named bla CTX-M-14a, and the remaining three isolates showed a nucleotide sequence identical to that of the blaCTX-M-9 gene except for one nucleotide, named blaCTX-M-14b. Characterisation of the regions surrounding the blaCTX-M-14a showed the ISEcp1 and the IS903 upstream and downstream, respectively, of the bla gene, whereas the regions surrounding the blaCTX-M-14b contained the genetic environment described for the blaCTX-M-9 gene, the In60. Characterisation by hybridisation showed that the blaCTX-M-14a was present in IncK plasmids, whereas the blaCTX-M-14b was found in the HI2 Inc group. The CTX-M-14 ESBL in E. coli isolates is the result of the convergence of two different genes. © 2007 Federation of European Microbiological Societies.

Original language	English
Pages (from-to)	120-123
Journal	FEMS Microbiology Letters
Volume	273
Issue number	1
DOIs	https://doi.org/10.1111/j.1574-6968.2007.00791.x
Publication status	Published - 1 Aug 2007

Keywords

CTX-M
CTX-M-14
ESBLs
Escherichia coli

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title = "Evidence for convergent evolution of CTX-M-14 ESBL in Escherichia coli and its prevalence",

abstract = "A total of 2440 Escherichia coli strains isolated in 2003 at the Hospital de la Santa Creu i Sant Pau were evaluated for the presence of extended-spectrum β-lactamases. Two different nucleotide sequences that encode the same β-lactamase, CTX-M-14, were detected when the blaCTX-M-14-genes of 35 E. coli isolates were analysed. Thirty-two of the 35 had the previously described sequence of the blaCTX-M-14 (AF252622), named bla CTX-M-14a, and the remaining three isolates showed a nucleotide sequence identical to that of the blaCTX-M-9 gene except for one nucleotide, named blaCTX-M-14b. Characterisation of the regions surrounding the blaCTX-M-14a showed the ISEcp1 and the IS903 upstream and downstream, respectively, of the bla gene, whereas the regions surrounding the blaCTX-M-14b contained the genetic environment described for the blaCTX-M-9 gene, the In60. Characterisation by hybridisation showed that the blaCTX-M-14a was present in IncK plasmids, whereas the blaCTX-M-14b was found in the HI2 Inc group. The CTX-M-14 ESBL in E. coli isolates is the result of the convergence of two different genes. {\textcopyright} 2007 Federation of European Microbiological Societies.",

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T1 - Evidence for convergent evolution of CTX-M-14 ESBL in Escherichia coli and its prevalence

AU - Navarro, Ferran

AU - Mesa, Rául Jesús

AU - Miró, Elisenda

AU - Gómez, Laura

AU - Mirelis, Beatriz

AU - Coll, Pere

PY - 2007/8/1

Y1 - 2007/8/1

N2 - A total of 2440 Escherichia coli strains isolated in 2003 at the Hospital de la Santa Creu i Sant Pau were evaluated for the presence of extended-spectrum β-lactamases. Two different nucleotide sequences that encode the same β-lactamase, CTX-M-14, were detected when the blaCTX-M-14-genes of 35 E. coli isolates were analysed. Thirty-two of the 35 had the previously described sequence of the blaCTX-M-14 (AF252622), named bla CTX-M-14a, and the remaining three isolates showed a nucleotide sequence identical to that of the blaCTX-M-9 gene except for one nucleotide, named blaCTX-M-14b. Characterisation of the regions surrounding the blaCTX-M-14a showed the ISEcp1 and the IS903 upstream and downstream, respectively, of the bla gene, whereas the regions surrounding the blaCTX-M-14b contained the genetic environment described for the blaCTX-M-9 gene, the In60. Characterisation by hybridisation showed that the blaCTX-M-14a was present in IncK plasmids, whereas the blaCTX-M-14b was found in the HI2 Inc group. The CTX-M-14 ESBL in E. coli isolates is the result of the convergence of two different genes. © 2007 Federation of European Microbiological Societies.

AB - A total of 2440 Escherichia coli strains isolated in 2003 at the Hospital de la Santa Creu i Sant Pau were evaluated for the presence of extended-spectrum β-lactamases. Two different nucleotide sequences that encode the same β-lactamase, CTX-M-14, were detected when the blaCTX-M-14-genes of 35 E. coli isolates were analysed. Thirty-two of the 35 had the previously described sequence of the blaCTX-M-14 (AF252622), named bla CTX-M-14a, and the remaining three isolates showed a nucleotide sequence identical to that of the blaCTX-M-9 gene except for one nucleotide, named blaCTX-M-14b. Characterisation of the regions surrounding the blaCTX-M-14a showed the ISEcp1 and the IS903 upstream and downstream, respectively, of the bla gene, whereas the regions surrounding the blaCTX-M-14b contained the genetic environment described for the blaCTX-M-9 gene, the In60. Characterisation by hybridisation showed that the blaCTX-M-14a was present in IncK plasmids, whereas the blaCTX-M-14b was found in the HI2 Inc group. The CTX-M-14 ESBL in E. coli isolates is the result of the convergence of two different genes. © 2007 Federation of European Microbiological Societies.

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KW - ESBLs

KW - Escherichia coli

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DO - 10.1111/j.1574-6968.2007.00791.x

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