Evidence for a specific regulation of recA gene transcription in Escherichia coli

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Abstract

The kinetics of the recA, sfiA and umuDC genes transcription were studied during a double SOS-inducting treatment in Escherichia coli cells using several strains carrying lacZ gene fusions. A transient inhibition in recA, but not in sfiA or umuDC promoted β-galactosidase synthesis was detected after successive UV-irradiations. Results obtained with a recA-lacZ fusion introduced in several DNA-repair mutants demonstrated that neither a lower LexA inactivation nor a decrease in the production of the inducing signal are the events through which the successive UV-irradiation promoted the arrest of recA transcription. On the contrary, a specific UV-dose-dependent delay appears to be the reason for the inhibition of the recA gene transcription in cells irradiated twice. © 1988.
Original languageEnglish
Pages (from-to)123-130
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume199
Issue number1
DOIs
Publication statusPublished - 1 Jan 1988

Keywords

  • Escherichia coli
  • Gene transcription, kinetics
  • RecA gene transcription
  • β-Galactosidase synthesis

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