Evaluation of two different cell lysis methods for releasing mycobacterial nucleic acids in the INNO-LiPA mycobacteria test

E. Padilla; V. González; J. M. Manterola; J. Lonca; A. Pérez; L. Matas; M. D. Quesada; V. Ausina

doi:10.1016/S0732-8893(03)00010-5

Evaluation of two different cell lysis methods for releasing mycobacterial nucleic acids in the INNO-LiPA mycobacteria test

E. Padilla, V. González, J. M. Manterola, J. Lonca, A. Pérez, L. Matas, M. D. Quesada, V. Ausina

Department of Genetics and Microbiology

Research output: Contribution to journal › Article › Research › peer-review

8 Citations (Scopus)

Abstract

The INNO-LiPA Mycobacteria Test (Innogenetics, N.V., Belgium) is a PCR-based reverse hybridization assay for the simultaneous identification of several mycobacterial species. We evaluated two simplified lysis methods for mycobacterial DNA release for application in the INNO-LiPA Mycobacteria Test. The two methods were based on either (i) heat treatment or (ii) sonication. Both methods were performed directly on 45 positive liquid cultures (MB-BacT, BioMérieux, Marcy l'Etoile, France) containing 17 different mycobacterial species. These two simple lysis procedures demonstrated similar effectiveness (100%) to that recommended by the manufacturer. They also significantly shortened the time required for mycobacterial DNA release. © 2003 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	19-23
Journal	Diagnostic Microbiology and Infectious Disease
Volume	46
Issue number	1
DOIs	https://doi.org/10.1016/S0732-8893(03)00010-5
Publication status	Published - 1 May 2003

Keywords

INNO-LiPA
Mycobacteria identification
PCR
Reverse hybridization

Access to Document

10.1016/S0732-8893(03)00010-5

Cite this

@article{b21cb736451e4e83951a7f334d684d1c,

title = "Evaluation of two different cell lysis methods for releasing mycobacterial nucleic acids in the INNO-LiPA mycobacteria test",

abstract = "The INNO-LiPA Mycobacteria Test (Innogenetics, N.V., Belgium) is a PCR-based reverse hybridization assay for the simultaneous identification of several mycobacterial species. We evaluated two simplified lysis methods for mycobacterial DNA release for application in the INNO-LiPA Mycobacteria Test. The two methods were based on either (i) heat treatment or (ii) sonication. Both methods were performed directly on 45 positive liquid cultures (MB-BacT, BioM{\'e}rieux, Marcy l'Etoile, France) containing 17 different mycobacterial species. These two simple lysis procedures demonstrated similar effectiveness (100%) to that recommended by the manufacturer. They also significantly shortened the time required for mycobacterial DNA release. {\textcopyright} 2003 Elsevier Inc. All rights reserved.",

keywords = "INNO-LiPA, Mycobacteria identification, PCR, Reverse hybridization",

author = "E. Padilla and V. Gonz{\'a}lez and Manterola, {J. M.} and J. Lonca and A. P{\'e}rez and L. Matas and Quesada, {M. D.} and V. Ausina",

year = "2003",

month = may,

day = "1",

doi = "10.1016/S0732-8893(03)00010-5",

language = "English",

volume = "46",

pages = "19--23",

journal = "Diagnostic Microbiology and Infectious Disease",

issn = "0732-8893",

number = "1",

}

TY - JOUR

T1 - Evaluation of two different cell lysis methods for releasing mycobacterial nucleic acids in the INNO-LiPA mycobacteria test

AU - Padilla, E.

AU - González, V.

AU - Manterola, J. M.

AU - Lonca, J.

AU - Pérez, A.

AU - Matas, L.

AU - Quesada, M. D.

AU - Ausina, V.

PY - 2003/5/1

Y1 - 2003/5/1

N2 - The INNO-LiPA Mycobacteria Test (Innogenetics, N.V., Belgium) is a PCR-based reverse hybridization assay for the simultaneous identification of several mycobacterial species. We evaluated two simplified lysis methods for mycobacterial DNA release for application in the INNO-LiPA Mycobacteria Test. The two methods were based on either (i) heat treatment or (ii) sonication. Both methods were performed directly on 45 positive liquid cultures (MB-BacT, BioMérieux, Marcy l'Etoile, France) containing 17 different mycobacterial species. These two simple lysis procedures demonstrated similar effectiveness (100%) to that recommended by the manufacturer. They also significantly shortened the time required for mycobacterial DNA release. © 2003 Elsevier Inc. All rights reserved.

AB - The INNO-LiPA Mycobacteria Test (Innogenetics, N.V., Belgium) is a PCR-based reverse hybridization assay for the simultaneous identification of several mycobacterial species. We evaluated two simplified lysis methods for mycobacterial DNA release for application in the INNO-LiPA Mycobacteria Test. The two methods were based on either (i) heat treatment or (ii) sonication. Both methods were performed directly on 45 positive liquid cultures (MB-BacT, BioMérieux, Marcy l'Etoile, France) containing 17 different mycobacterial species. These two simple lysis procedures demonstrated similar effectiveness (100%) to that recommended by the manufacturer. They also significantly shortened the time required for mycobacterial DNA release. © 2003 Elsevier Inc. All rights reserved.

KW - INNO-LiPA

KW - Mycobacteria identification

KW - PCR

KW - Reverse hybridization

U2 - 10.1016/S0732-8893(03)00010-5

DO - 10.1016/S0732-8893(03)00010-5

M3 - Article

SN - 0732-8893

VL - 46

SP - 19

EP - 23

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 1

ER -

Evaluation of two different cell lysis methods for releasing mycobacterial nucleic acids in the INNO-LiPA mycobacteria test

Abstract

Keywords

Access to Document

Fingerprint

Cite this