Evaluation of two different cell lysis methods for releasing mycobacterial nucleic acids in the INNO-LiPA mycobacteria test

E. Padilla, V. González, J. M. Manterola, J. Lonca, A. Pérez, L. Matas, M. D. Quesada, V. Ausina

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

The INNO-LiPA Mycobacteria Test (Innogenetics, N.V., Belgium) is a PCR-based reverse hybridization assay for the simultaneous identification of several mycobacterial species. We evaluated two simplified lysis methods for mycobacterial DNA release for application in the INNO-LiPA Mycobacteria Test. The two methods were based on either (i) heat treatment or (ii) sonication. Both methods were performed directly on 45 positive liquid cultures (MB-BacT, BioMérieux, Marcy l'Etoile, France) containing 17 different mycobacterial species. These two simple lysis procedures demonstrated similar effectiveness (100%) to that recommended by the manufacturer. They also significantly shortened the time required for mycobacterial DNA release. © 2003 Elsevier Inc. All rights reserved.
Original languageEnglish
Pages (from-to)19-23
JournalDiagnostic Microbiology and Infectious Disease
Volume46
Issue number1
DOIs
Publication statusPublished - 1 May 2003

Keywords

  • INNO-LiPA
  • Mycobacteria identification
  • PCR
  • Reverse hybridization

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