TY - JOUR
T1 - Evaluation of the SediMax automated microscopy sediment analyzer and the Sysmex UF-1000i flow cytometer as screening tools to rule out negative urinary tract infections
AU - Íñigo, Melania
AU - Coello, Andreu
AU - Fernández-Rivas, Gema
AU - Carrasco, María
AU - Marcó, Clara
AU - Fernández, Anabel
AU - Casamajor, Teresa
AU - Ausina, Vicente
PY - 2016/5/1
Y1 - 2016/5/1
N2 - © 2016 Elsevier B.V.. Urinary tract infections (UTI) are highly prevalent in nosocomial and community settings, and their diagnosis is costly and time-consuming. Screening methods represent an important advance towards the final UTI diagnosis, diminishing inappropriate treatment or clinical complications. Automated analyzers have been developed and commercialized to screen and rule out negative urine samples. The aim of this study was to evaluate two of these automated analyzers (SediMax, an automatic sediment analyzer and UF-1000i a flow cytometer) to predict negative urine cultures. A total of 1934 urine samples were analyzed. A very strong correlation for white blood cells (WBC) (r s : 0.928) and a strong correlation for bacteria (BAC) (r s : 0.693) were obtained. We also calculated optimal cut-off points for both autoanalyzers: 18 WBC/μL and 97 BAC/μL for SediMax (sensitivity = 96.25%, specificity = 63.04%, negative predictive value = 97.97%), and 40 WBC/μL and 460 BAC/μL for UF-1000i (sensitivity = 98.13%, specificity = 79.16%, negative predictive value = 99.18%). The use of SediMax and UF-1000i resulted in a 46.33% and 57.19% reduction of all samples cultured, respectively. In conclusion, both analyzers are good UTI screening tools in our setting.
AB - © 2016 Elsevier B.V.. Urinary tract infections (UTI) are highly prevalent in nosocomial and community settings, and their diagnosis is costly and time-consuming. Screening methods represent an important advance towards the final UTI diagnosis, diminishing inappropriate treatment or clinical complications. Automated analyzers have been developed and commercialized to screen and rule out negative urine samples. The aim of this study was to evaluate two of these automated analyzers (SediMax, an automatic sediment analyzer and UF-1000i a flow cytometer) to predict negative urine cultures. A total of 1934 urine samples were analyzed. A very strong correlation for white blood cells (WBC) (r s : 0.928) and a strong correlation for bacteria (BAC) (r s : 0.693) were obtained. We also calculated optimal cut-off points for both autoanalyzers: 18 WBC/μL and 97 BAC/μL for SediMax (sensitivity = 96.25%, specificity = 63.04%, negative predictive value = 97.97%), and 40 WBC/μL and 460 BAC/μL for UF-1000i (sensitivity = 98.13%, specificity = 79.16%, negative predictive value = 99.18%). The use of SediMax and UF-1000i resulted in a 46.33% and 57.19% reduction of all samples cultured, respectively. In conclusion, both analyzers are good UTI screening tools in our setting.
KW - Autoanalyzers
KW - Automated urine sediment
KW - Flow cytometry
KW - Urinalysis
KW - Urinary tract infection
U2 - 10.1016/j.cca.2016.02.016
DO - 10.1016/j.cca.2016.02.016
M3 - Article
SN - 0009-8981
VL - 456
SP - 31
EP - 35
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
ER -