Evaluation of the effect of some nonionic detergents and diazonium salts on the determination of acid phosphatase activity in sera

R. Ferragut Mas, F. Canalias Reverter, F. J. Gella Tomas

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The effect of several nonionic detergents and diazonium salts on the continuous monitoring method for acid phosphatase determination using 1-naphthyl phosphate as substrate has been studied. It was found that detergents improved the stability of both reagent and diazocomplex formed between diazonium salt and 1-naphtol. Nonidet® P-40 at 2 g/L concentration was selected as the most effective detergent. On the other hand, it was found that Fast Red ITR salt also improved both reagent and diazocomplex stability than to commonly used Fast Red TR salt. The sensitivity of the assay was not affected.
Original languageEnglish
Pages (from-to)212-216
JournalRevista de la Sociedad Espanola de Quimica Clinica
Volume15
Issue number4
Publication statusPublished - 1 Jan 1996

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