Evaluation of the effect of some nonionic detergents and diazonium salts on the determination of acid phosphatase activity in sera

R. Ferragut Mas; F. Canalias Reverter; F. J. Gella Tomas

Evaluation of the effect of some nonionic detergents and diazonium salts on the determination of acid phosphatase activity in sera

R. Ferragut Mas, F. Canalias Reverter, F. J. Gella Tomas

Department of Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › Research › peer-review

Abstract

The effect of several nonionic detergents and diazonium salts on the continuous monitoring method for acid phosphatase determination using 1-naphthyl phosphate as substrate has been studied. It was found that detergents improved the stability of both reagent and diazocomplex formed between diazonium salt and 1-naphtol. Nonidet® P-40 at 2 g/L concentration was selected as the most effective detergent. On the other hand, it was found that Fast Red ITR salt also improved both reagent and diazocomplex stability than to commonly used Fast Red TR salt. The sensitivity of the assay was not affected.

Original language	English
Pages (from-to)	212-216
Journal	Revista de la Sociedad Espanola de Quimica Clinica
Volume	15
Issue number	4
Publication status	Published - 1 Jan 1996

Cite this

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title = "Evaluation of the effect of some nonionic detergents and diazonium salts on the determination of acid phosphatase activity in sera",

abstract = "The effect of several nonionic detergents and diazonium salts on the continuous monitoring method for acid phosphatase determination using 1-naphthyl phosphate as substrate has been studied. It was found that detergents improved the stability of both reagent and diazocomplex formed between diazonium salt and 1-naphtol. Nonidet{\textregistered} P-40 at 2 g/L concentration was selected as the most effective detergent. On the other hand, it was found that Fast Red ITR salt also improved both reagent and diazocomplex stability than to commonly used Fast Red TR salt. The sensitivity of the assay was not affected.",

author = "{Ferragut Mas}, R. and {Canalias Reverter}, F. and {Gella Tomas}, {F. J.}",

year = "1996",

month = jan,

day = "1",

language = "English",

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pages = "212--216",

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Evaluation of the effect of some nonionic detergents and diazonium salts on the determination of acid phosphatase activity in sera. / Ferragut Mas, R.; Canalias Reverter, F.; Gella Tomas, F. J.

In: Revista de la Sociedad Espanola de Quimica Clinica, Vol. 15, No. 4, 01.01.1996, p. 212-216.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Evaluation of the effect of some nonionic detergents and diazonium salts on the determination of acid phosphatase activity in sera

AU - Ferragut Mas, R.

AU - Canalias Reverter, F.

AU - Gella Tomas, F. J.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - The effect of several nonionic detergents and diazonium salts on the continuous monitoring method for acid phosphatase determination using 1-naphthyl phosphate as substrate has been studied. It was found that detergents improved the stability of both reagent and diazocomplex formed between diazonium salt and 1-naphtol. Nonidet® P-40 at 2 g/L concentration was selected as the most effective detergent. On the other hand, it was found that Fast Red ITR salt also improved both reagent and diazocomplex stability than to commonly used Fast Red TR salt. The sensitivity of the assay was not affected.

AB - The effect of several nonionic detergents and diazonium salts on the continuous monitoring method for acid phosphatase determination using 1-naphthyl phosphate as substrate has been studied. It was found that detergents improved the stability of both reagent and diazocomplex formed between diazonium salt and 1-naphtol. Nonidet® P-40 at 2 g/L concentration was selected as the most effective detergent. On the other hand, it was found that Fast Red ITR salt also improved both reagent and diazocomplex stability than to commonly used Fast Red TR salt. The sensitivity of the assay was not affected.

M3 - Article

VL - 15

SP - 212

EP - 216

IS - 4

ER -

Evaluation of the effect of some nonionic detergents and diazonium salts on the determination of acid phosphatase activity in sera

Abstract

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