Evaluation of the BacT/ALERT PZA kit in comparison with the BACTEC 460TB PZA for testing Mycobacterium tuberculosis susceptibility to pyrazinamide

Lina Marcela Aragón; Montserrat Garrigó; Carmen Moreno; Montserrat Español; Pere Coll

doi:10.1093/jac/dkm252

Evaluation of the BacT/ALERT PZA kit in comparison with the BACTEC 460TB PZA for testing Mycobacterium tuberculosis susceptibility to pyrazinamide

Lina Marcela Aragón, Montserrat Garrigó, Carmen Moreno, Montserrat Español, Pere Coll

Department of Genetics and Microbiology

Research output: Contribution to journal › Article › Research › peer-review

7 Citations (Scopus)

Abstract

Objectives: To compare the performance of the BacT/ALERT PZA kit (BioMerieux, Marcy l'Etoile, France) with the radiometric BACTEC 460TB PZA test (Becton-Dickinson method) for testing Mycobacterium tuberculosis susceptibility to pyrazinamide. Methods: A total of 50 M. tuberculosis strains were tested. Thirty of these strains had been previously considered pyrazinamide-susceptible and 20 pyrazinamide-resistant by BACTEC 460TB. Results: Final overall agreement was 100%. Time needed for the susceptibility test was 6.69 days for the BacT/ALERT PZA kit versus 8.07 days for the BACTEC 460TB PZA test. Conclusions: BacT/ALERT PZA test isan excellent alternative to BACTEC 460TB for pyrazinamide susceptibility testing. © The Author 2007. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.

Original language	English
Pages (from-to)	655-657
Journal	Journal of Antimicrobial Chemotherapy
Volume	60
Issue number	3
DOIs	https://doi.org/10.1093/jac/dkm252
Publication status	Published - 1 Sep 2007

Keywords

Resistance
Susceptibility testing
Tuberculosis

UN SDGs

This output contributes to the following Sustainable Development Goal(s)

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@article{e65543ecb35e4fe68856a48b668ecc10,

title = "Evaluation of the BacT/ALERT PZA kit in comparison with the BACTEC 460TB PZA for testing Mycobacterium tuberculosis susceptibility to pyrazinamide",

abstract = "Objectives: To compare the performance of the BacT/ALERT PZA kit (BioMerieux, Marcy l'Etoile, France) with the radiometric BACTEC 460TB PZA test (Becton-Dickinson method) for testing Mycobacterium tuberculosis susceptibility to pyrazinamide. Methods: A total of 50 M. tuberculosis strains were tested. Thirty of these strains had been previously considered pyrazinamide-susceptible and 20 pyrazinamide-resistant by BACTEC 460TB. Results: Final overall agreement was 100%. Time needed for the susceptibility test was 6.69 days for the BacT/ALERT PZA kit versus 8.07 days for the BACTEC 460TB PZA test. Conclusions: BacT/ALERT PZA test isan excellent alternative to BACTEC 460TB for pyrazinamide susceptibility testing. {\textcopyright} The Author 2007. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.",

keywords = "Resistance, Susceptibility testing, Tuberculosis",

author = "Arag{\'o}n, {Lina Marcela} and Montserrat Garrig{\'o} and Carmen Moreno and Montserrat Espa{\~n}ol and Pere Coll",

year = "2007",

month = sep,

day = "1",

doi = "10.1093/jac/dkm252",

language = "English",

volume = "60",

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Evaluation of the BacT/ALERT PZA kit in comparison with the BACTEC 460TB PZA for testing Mycobacterium tuberculosis susceptibility to pyrazinamide. / Aragón, Lina Marcela; Garrigó, Montserrat; Moreno, Carmen; Español, Montserrat; Coll, Pere.

In: Journal of Antimicrobial Chemotherapy, Vol. 60, No. 3, 01.09.2007, p. 655-657.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Evaluation of the BacT/ALERT PZA kit in comparison with the BACTEC 460TB PZA for testing Mycobacterium tuberculosis susceptibility to pyrazinamide

AU - Aragón, Lina Marcela

AU - Garrigó, Montserrat

AU - Moreno, Carmen

AU - Español, Montserrat

AU - Coll, Pere

PY - 2007/9/1

Y1 - 2007/9/1

N2 - Objectives: To compare the performance of the BacT/ALERT PZA kit (BioMerieux, Marcy l'Etoile, France) with the radiometric BACTEC 460TB PZA test (Becton-Dickinson method) for testing Mycobacterium tuberculosis susceptibility to pyrazinamide. Methods: A total of 50 M. tuberculosis strains were tested. Thirty of these strains had been previously considered pyrazinamide-susceptible and 20 pyrazinamide-resistant by BACTEC 460TB. Results: Final overall agreement was 100%. Time needed for the susceptibility test was 6.69 days for the BacT/ALERT PZA kit versus 8.07 days for the BACTEC 460TB PZA test. Conclusions: BacT/ALERT PZA test isan excellent alternative to BACTEC 460TB for pyrazinamide susceptibility testing. © The Author 2007. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.

AB - Objectives: To compare the performance of the BacT/ALERT PZA kit (BioMerieux, Marcy l'Etoile, France) with the radiometric BACTEC 460TB PZA test (Becton-Dickinson method) for testing Mycobacterium tuberculosis susceptibility to pyrazinamide. Methods: A total of 50 M. tuberculosis strains were tested. Thirty of these strains had been previously considered pyrazinamide-susceptible and 20 pyrazinamide-resistant by BACTEC 460TB. Results: Final overall agreement was 100%. Time needed for the susceptibility test was 6.69 days for the BacT/ALERT PZA kit versus 8.07 days for the BACTEC 460TB PZA test. Conclusions: BacT/ALERT PZA test isan excellent alternative to BACTEC 460TB for pyrazinamide susceptibility testing. © The Author 2007. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.

KW - Resistance

KW - Susceptibility testing

KW - Tuberculosis

U2 - 10.1093/jac/dkm252

DO - 10.1093/jac/dkm252

M3 - Article

VL - 60

SP - 655

EP - 657

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ER -