TY - JOUR
T1 - Evaluation of histidine decarboxylase activity of bacteria isolated from sardine (Sardina pilchardus) by an enzymic method
AU - López‐Sabater, E. I.
AU - Rodriguez‐Jerez, J. J.
AU - Hernández‐Herrero, M.
AU - Mora‐Ventura, M. T.
PY - 1994/1/1
Y1 - 1994/1/1
N2 - A fast and simple enzymic method has been adapted to measure histidine decarboxylase activity in bacteria isolated from fish. It was possible to quantify the presence of histamine at levels higher than 1.03 times 10‐2μmol (3 ppm) per ml of culture medium. There was a good correlation (γ=0.99) between the histamine content and the increase of absorbance in the concentration range of 3 and 30 ppm. The method can be used for quantitation of the amount of histamine produced by a selected strain, and has the advantages of being low in cost, reliable, easy to use and fast to perform. Plesiomonas shigelloides, a bacterium frequently isolated from fish and aquatic environments, has been identified as a new histamine‐former in fish. Copyright © 1994, Wiley Blackwell. All rights reserved
AB - A fast and simple enzymic method has been adapted to measure histidine decarboxylase activity in bacteria isolated from fish. It was possible to quantify the presence of histamine at levels higher than 1.03 times 10‐2μmol (3 ppm) per ml of culture medium. There was a good correlation (γ=0.99) between the histamine content and the increase of absorbance in the concentration range of 3 and 30 ppm. The method can be used for quantitation of the amount of histamine produced by a selected strain, and has the advantages of being low in cost, reliable, easy to use and fast to perform. Plesiomonas shigelloides, a bacterium frequently isolated from fish and aquatic environments, has been identified as a new histamine‐former in fish. Copyright © 1994, Wiley Blackwell. All rights reserved
U2 - 10.1111/j.1472-765X.1994.tb00908.x
DO - 10.1111/j.1472-765X.1994.tb00908.x
M3 - Article
VL - 19
SP - 70
EP - 75
JO - Letters in Applied Microbiology
JF - Letters in Applied Microbiology
SN - 0266-8254
IS - 2
ER -