Evaluation of cell-mediated immune responses against porcine circovirus type 2 (PCV2) Cap and Rep proteins after vaccination with a commercial PCV2 sub-unit vaccine

Maria Fort, Marina Sibila, Miquel Nofrarías, Eva Pérez-Martín, Alex Olvera, Enric Mateu, Joaquim Segalés

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13 Citations (Scopus)

Abstract

This study investigated the development of cellular immunity to Porcine circovirus type 2 (PCV2) Cap and Rep proteins in pigs vaccinated with a commercial PCV2 genotype a (PCV2a) based sub-unit vaccine, before and after a heterologous challenge with a PCV2b isolate. At three weeks of age, 20 pigs were inoculated intramuscularly with either the vaccine product (V group, n= 9) or phosphate buffered saline solution (PBS) (NV group, n= 11). Three weeks after vaccination, pigs were challenged intranasally with PCV2b (V-C and NV-C groups) or PBS (V-NC and NV-NC groups). None of the pigs developed clinical signs during the whole experiment, but all NV-C and 3/5 V-C pigs developed viraemia. Vaccination induced the development IFN-γ-secreting cells in response to the Cap protein of PCV2, which appeared three weeks post-vaccination and increased after challenge. By that time, no significant differences were detected on PCV2 antibody titres between vaccinated and non-vaccinated pigs, although there were significant differences on day 7 post-challenge. PCV2-inoculation induced a cellular response against the Rep protein. Such response was significantly reduced or even absent in PCV2-inoculated pigs that were previously vaccinated (V-C group), presumably as a result of a lower PCV2 replication in vaccinated animals compared to non-vaccinated ones. © 2012 Elsevier B.V.
Original languageEnglish
Pages (from-to)128-132
JournalVeterinary Immunology and Immunopathology
Volume150
Issue number1-2
DOIs
Publication statusPublished - 15 Nov 2012

Keywords

  • Capsid protein
  • Cellular immunity
  • Humoral immunity
  • Porcine circovirus type 2 (PCV2)
  • Replicase protein
  • Vaccine

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