Evaluation of an antibody avidity index method for detecting recent human immunodeficiency virus type 1 infection using an automated chemiluminescence immunoassay

Gema Fernández, Christian Manzardo, Alexandra Montoliu, Colin Campbell, Gregorio Fernández, Jordi Casabona, José Maria Miró, Lurdes Matas, Belén Rivaya, Victoria González

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1 Citation (Scopus)

Abstract

© 2014 Elsevier Espanã, S.L.U. y Sociedad Española de Enfermedades Infecciosas y Microbiología Cli;acutenica. Background Recent infection testing algorithms (RITAs) are used in public health surveillance to estimate the incidence of recently acquired HIV-1 infection. Objectives Our aims were (i) to evaluate the precision of the VITROS<sup>®</sup> Anti-HIV 1+2 automated antibody avidity assay for qualitative detection of antibodies to HIV 1+2 virus; (ii) to validate the accuracy of an automated guanidine-based antibody avidity assay to discriminate between recent and long standing infections using the VITROS 3600 platform; (iii) to compare this method with BED-CEIA assay; and (iv) to evaluate the occurrence of false recent misclassifications by the VITROS antibody avidity assay in patients with a CD4 count <200 cells/μL and in patients on combination antiretroviral therapy (cART). Results The VITROS<sup>®</sup> antibody avidity assay is highly reproducible. The ROC curve analysis of the accuracy of this assay, optimized for sensitivity and specificity, had an AI cut off of ≥0.51, with sensitivity and specificity values of 86.67% (95% CI: 72.51-94.46) and 86.24% (95% CI: 78.00-91.84), respectively. The agreement between VITROS antibody avidity and BED-CEIA assays was good. Misclassifications of long standing infections as recent infection occurred in 8.2% of patients with CD4 <200 cell/μL and 8.7% in patients on combination antiretroviral therapy. Conclusions The VITROS antibody avidity assay is a reliable serological method to detect recent HIV-1 infections and it could be incorporated into a RITA to estimate HIV incidence.
Original languageEnglish
Pages (from-to)238-242
JournalEnfermedades Infecciosas y Microbiologia Clinica
Volume33
Issue number4
DOIs
Publication statusPublished - 1 Jan 2015

Keywords

  • Avidity index
  • False recent rate
  • Human immunodeficiency virus
  • Incidence
  • Recent infection

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